Loss-of-function mutations of the rice GAMYB gene impair α-amylase expression in aleurone and flower development

被引:251
作者
Kaneko, M
Inukai, Y
Ueguchi-Tanaka, M
Itoh, H
Izawa, T
Kobayashi, Y
Hattori, T
Miyao, A
Hirochika, H
Ashikari, M
Matsuoka, M [1 ]
机构
[1] Nagoya Univ, BioSci Ctr, Nagoya, Aichi 4648601, Japan
[2] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan
关键词
D O I
10.1105/tpc.017327
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
GAMYB was first isolated as a positive transcriptional regulator of gibberellin (GA)-dependent alpha-amylase expression in barley aleurone cells, and its molecular and biochemical properties have been well characterized. However, the role of GAMYB elsewhere in the plant is not well understood. To investigate the molecular function of GAMYB outside of the aleurone cells, we isolated loss-of-function mutants from a panel of rice mutants produced by the insertion of a retrotransposon, Tos17. Through PCR screening using primers for rice GAMYB (OsGAMYB) and Tos17, we isolated three independent mutant alleles that contained Tos17 inserted in the exon region. No alpha-amylase expression in the endosperm was induced in these mutants in response to GA treatment, indicating that the Tos17 insertion had knocked out OsGAMYB function. We found no significant defects in the growth and development of the mutants at the vegetative stage. After the phase transition to the reproductive stage, however, shortened internodes and defects in floral organ development, especially a defect in pollen development, were observed. On the other hand, no difference was detected in flowering time. High-level OsGAMYB expression was detected in the aleurone cells, inflorescence shoot apical region, stamen primordia, and tapetum cells of the anther, but only low-level expression occurred in organs at the vegetative stage or in the elongating stem. These results demonstrate that, in addition to its role in the induction of alpha-amylase in aleurone, OsGAMYB also is important for floral organ development and essential for pollen development.
引用
收藏
页码:33 / 44
页数:12
相关论文
共 40 条
[1]   Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter [J].
Blázquez, MA ;
Green, R ;
Nilsson, O ;
Sussman, MR ;
Weigel, D .
PLANT CELL, 1998, 10 (05) :791-800
[2]   Integration of floral inductive signals in Arabidopsis [J].
Blázquez, MA ;
Weigel, D .
NATURE, 2000, 404 (6780) :889-892
[3]   A variety of wheat tolerant to deep-seeding conditions: elongation of the first internode depends on the response to gibberellin and potassium [J].
Chen, L ;
Nishizawa, T ;
Higashitani, A ;
Suge, H ;
Wakui, Y ;
Takeda, K ;
Takahashi, H .
PLANT CELL AND ENVIRONMENT, 2001, 24 (04) :469-476
[4]  
Davies PJ, 1995, PLANT HORMONES PHYSL
[5]  
EVENS LT, 1990, PLANTA, V182, P97
[6]   Sequence and analysis of rice chromosome 4 [J].
Feng, Q ;
Zhang, YJ ;
Hao, P ;
Wang, SY ;
Fu, G ;
Huang, YC ;
Li, Y ;
Zhu, JJ ;
Liu, YL ;
Hu, X ;
Jia, PX ;
Zhang, Y ;
Zhao, Q ;
Ying, K ;
Yu, SL ;
Tang, YS ;
Weng, QJ ;
Zhang, L ;
Lu, Y ;
Mu, J ;
Lu, YQ ;
Zhang, LS ;
Yu, Z ;
Fan, DL ;
Liu, XH ;
Lu, TT ;
Li, C ;
Wu, YR ;
Sun, TG ;
Lei, HY ;
Li, T ;
Hu, H ;
Guan, JP ;
Wu, M ;
Zhang, RQ ;
Zhou, B ;
Chen, ZH ;
Chen, L ;
Jin, ZQ ;
Wang, R ;
Yin, HF ;
Cai, Z ;
Ren, SX ;
Lv, G ;
Gu, WY ;
Zhu, GF ;
Tu, YF ;
Jia, J ;
Zhang, Y ;
Chen, J .
NATURE, 2002, 420 (6913) :316-320
[7]   Long-day up-regulation of a GAMYB gene during Lolium temulentum inflorescence formation [J].
Gocal, GFW ;
Poole, AT ;
Gubler, F ;
Watts, RJ ;
Blundell, C ;
King, RW .
PLANT PHYSIOLOGY, 1999, 119 (04) :1271-1278
[8]  
Gocal GFW, 2001, PLANT PHYSIOL, V127, P1682, DOI 10.1104/pp.010442
[9]   A draft sequence of the rice genome (Oryza sativa L. ssp japonica) [J].
Goff, SA ;
Ricke, D ;
Lan, TH ;
Presting, G ;
Wang, RL ;
Dunn, M ;
Glazebrook, J ;
Sessions, A ;
Oeller, P ;
Varma, H ;
Hadley, D ;
Hutchinson, D ;
Martin, C ;
Katagiri, F ;
Lange, BM ;
Moughamer, T ;
Xia, Y ;
Budworth, P ;
Zhong, JP ;
Miguel, T ;
Paszkowski, U ;
Zhang, SP ;
Colbert, M ;
Sun, WL ;
Chen, LL ;
Cooper, B ;
Park, S ;
Wood, TC ;
Mao, L ;
Quail, P ;
Wing, R ;
Dean, R ;
Yu, YS ;
Zharkikh, A ;
Shen, R ;
Sahasrabudhe, S ;
Thomas, A ;
Cannings, R ;
Gutin, A ;
Pruss, D ;
Reid, J ;
Tavtigian, S ;
Mitchell, J ;
Eldredge, G ;
Scholl, T ;
Miller, RM ;
Bhatnagar, S ;
Adey, N ;
Rubano, T ;
Tusneem, N .
SCIENCE, 2002, 296 (5565) :92-100
[10]   Cloning of a rice cDNA encoding a transcription factor homologous to barley GAMyb [J].
Gubler, F ;
Watts, RJ ;
Kalla, R ;
Matthews, P ;
Keys, M ;
Jacobsen, JV .
PLANT AND CELL PHYSIOLOGY, 1997, 38 (03) :362-365