Expression of MUC2 and MUC3 mRNA in human normal, malignant, and inflammatory intestinal tissues

被引:111
作者
Weiss, AA [1 ]
Babyatsky, MW [1 ]
Ogata, S [1 ]
Chen, A [1 ]
Itzkowitz, SH [1 ]
机构
[1] CUNY MT SINAI SCH MED,GASTROINTESTINAL RES LAB,DEPT MED,NEW YORK,NY 10029
关键词
mucin genes; human intestine; MUC2; MUC3; in situ hybridization; colon cancer; inflammatory bowel disease; ulcerative colitis; Crohn's disease;
D O I
10.1177/44.10.8813081
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
MUC2 and MUC3 are prominent mucin genes expressed in the human intestine. Using in situ hybridization with RNA probes, we examined the cellular distribution of MUC2 and MUC3 mRNA in normal, malignant, and inflammatory human intestinal tissues. In normal small intestine and colon. MUC2 mRNA was expressed exclusively in goblet cells and occurred throughout the entire height of the mucosa. MUC3 mRNA was expressed by goblet and columnar cells but was restricted to the villous compartment of the small intestine and the surface epithelium of the colon. Expression of MUC2 and MUC3 mRNA were both markedly decreased in poorly, moderately, and well-differentiated colon cancers but were preserved in mucinous colon cancers. In ulcerative colitis and Crohn's colitis tissues, MUC2 and MUC3 mRNA expression displayed a normal pattern regardless of whether the mucosa manifested active or quiescent inflammation. These findings indicate that MUC2 is goblet cell-specific, whereas MUC3 is related to maturation of intestinal epithelial cells. In colon cancers, the genetic regulation of MUC2 and MUC3 is different depending on the histological type of tumor. The constitutive expression of MC2 and MUC3 mRNA in inflammatory bowel diseases suggests that these genes may be necessary for maintenance of normal epithelial cell function during inflammation.
引用
收藏
页码:1161 / 1166
页数:6
相关论文
共 27 条
[21]  
PODOLSKY DK, 1984, GASTROENTEROLOGY, V87, P991
[22]   ION-EXCHANGE CHROMATOGRAPHY OF PURIFIED COLONIC MUCUS GLYCOPROTEINS IN INFLAMMATORY BOWEL-DISEASE - ABSENCE OF A SELECTIVE SUBCLASS DEFECT [J].
RAOUF, A ;
PARKER, N ;
IDDON, D ;
RYDER, S ;
LANGDONBROWN, B ;
MILTON, JD ;
WALKER, R ;
RHODES, JM .
GUT, 1991, 32 (10) :1139-1145
[23]  
SAMBROOK J, 1989, MOL CLONING LAB MANU, V3
[24]   COLONIC GLYCOPROTEINS IN MONOZYGOTIC TWINS WITH INFLAMMATORY BOWEL-DISEASE [J].
TYSK, C ;
RIEDESEL, H ;
LINDBERG, E ;
PANZINI, B ;
PODOLSKY, D ;
JARNEROT, G .
GASTROENTEROLOGY, 1991, 100 (02) :419-423
[25]   BIOSYNTHESIS OF HUMAN COLONIC MUCIN - MUC2 IS THE PROMINENT SECRETORY MUCIN [J].
TYTGAT, KMAJ ;
BULLER, HA ;
OPDAM, FJM ;
KIM, YS ;
EINERHAND, AWC ;
DEKKER, J .
GASTROENTEROLOGY, 1994, 107 (05) :1352-1363
[26]   MUC2 is the prominent colonic mucin expressed in ulcerative colitis1 [J].
Tytgat, KMAJ ;
Opdam, FJM ;
Einerhand, AWC ;
Buller, HA ;
Dekker, J .
GUT, 1996, 38 (04) :554-563
[27]  
ZOTTER S, 1988, Cancer Reviews, V11-12, P55