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Peptide biomarkers as a way to determine meat authenticity
被引:57
作者:
Angel Sentandreu, Miguel
[1
]
Sentandreu, Enrique
[1
]
机构:
[1] CSIC, Inst Agroquim & Tecnol Alimentos, Valencia 46980, Spain
关键词:
Peptide biomarkers;
Meat authentication;
Proteomics;
Mass spectrometry;
MASS-SPECTROMETRY;
SPECIES IDENTIFICATION;
POSTMORTEM PROTEOLYSIS;
LIQUID-CHROMATOGRAPHY;
WATER DISTRIBUTION;
PROTEOMIC APPROACH;
MUSCLE PROTEOME;
PORCINE MUSCLE;
FOOD FORENSICS;
PROTEINS;
D O I:
10.1016/j.meatsci.2011.04.028
中图分类号:
TS2 [食品工业];
学科分类号:
0832 ;
摘要:
Meat fraud implies many illegal procedures affecting the composition of meat and meat products, something that is commonly done with the aim to increase profit. These practices need to be controlled by legal authorities by means of robust, accurate and sensitive methodologies capable to assure that fraudulent or accidental mislabelling does not arise. Common strategies traditionally used to assess meat authenticity have been based on methods such as chemometric analysis of a large set of data analysis, immunoassays or DNA analysis. The identification of peptide biomarkers specific of a particular meat species, tissue or ingredient by proteomic technologies constitutes an interesting and promising alternative to existing methodologies due to its high discriminating power, robustness and sensitivity. The possibility to develop standardized protein extraction protocols, together with the considerably higher resistance of peptide sequences to food processing as compared to DNA sequences, would overcome some of the limitations currently existing for quantitative determinations of highly processed food samples. The use of routine mass spectrometry equipment would make the technology suitable for control laboratories. (C) 2011 Elsevier Ltd. All rights reserved.
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页码:280 / 285
页数:6
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