A major protein component of the Bacillus subtilis biofilm matrix

被引:511
作者
Branda, SS
Chu, F
Kearns, DB
Losick, R
Kolter, R [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
[2] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
关键词
D O I
10.1111/j.1365-2958.2005.05020.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microbes construct structurally complex multicellular communities (biofilms) through production of an extracellular matrix. Here we present evidence from scanning electron microscopy showing that a wild strain of the Gram positive bacterium Bacillus subtilis builds such a matrix. Genetic, biochemical and cytological evidence indicates that the matrix is composed predominantly of a protein component, TasA, and an exopolysaccharide component. The absence of TasA or the exopolysaccharide resulted in a residual matrix, while the absence of both components led to complete failure to form complex multicellular communities. Extracellular complementation experiments revealed that a functional matrix can be assembled even when TasA and the exopolysaccharide are produced by different cells, reinforcing the view that the components contribute to matrix formation in an extracellular manner. Having defined the major components of the biofilm matrix and the control of their synthesis by the global regulator SinR, we present a working model for how B. subtilis switches between nomadic and sedentary lifestyles.
引用
收藏
页码:1229 / 1238
页数:10
相关论文
共 31 条
  • [1] A proteomic view on genome-based signal peptide predictions
    Antelmann, H
    Tjalsma, H
    Voigt, B
    Ohlmeier, S
    Bron, S
    van Dijl, JM
    Hecker, M
    [J]. GENOME RESEARCH, 2001, 11 (09) : 1484 - 1502
  • [2] A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336:: Gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells
    Ashiuchi, M
    Soda, K
    Misono, H
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 263 (01) : 6 - 12
  • [3] Physiological and biochemical characteristics of poly γ-glutamate synthetase complex of Bacillus subtilis
    Ashiuchi, M
    Nawa, C
    Kamei, T
    Song, JJ
    Hong, SP
    Sung, MH
    Soda, K
    Yagi, T
    Misono, H
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (20): : 5321 - 5328
  • [4] COUPLING OF FLAGELLIN GENE-TRANSCRIPTION TO FLAGELLAR ASSEMBLY IN BACILLUS-SUBTILIS
    BARILLA, D
    CARAMORI, T
    GALIZZI, A
    [J]. JOURNAL OF BACTERIOLOGY, 1994, 176 (15) : 4558 - 4564
  • [5] Biofilms:: the matrix revisited
    Branda, SS
    Vik, Å
    Friedman, L
    Kolter, R
    [J]. TRENDS IN MICROBIOLOGY, 2005, 13 (01) : 20 - 26
  • [6] Genes involved in formation of structured multicellular communities by Bacillus subtilis
    Branda, SS
    González-Pastor, JE
    Dervyn, E
    Ehrlich, SD
    Losick, R
    Kolter, R
    [J]. JOURNAL OF BACTERIOLOGY, 2004, 186 (12) : 3970 - 3979
  • [7] Fruiting body formation by Bacillus subtilis
    Branda, SS
    González-Pastor, JE
    Ben-Yehuda, S
    Losick, R
    Kolter, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) : 11621 - 11626
  • [8] CHU F, 2006, IN PRESS MOL MICRO, DOI DOI 10.1111/J.1365-2958.2005.050189.X
  • [9] Developmental commitment in a bacterium
    Dworkin, J
    Losick, R
    [J]. CELL, 2005, 121 (03) : 401 - 409
  • [10] FlgM is a primary regulator of sigma(D) activity, and its absence restores motility to a sinR mutant
    Fredrick, K
    Helmann, JD
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (23) : 7010 - 7013