A major protein component of the Bacillus subtilis biofilm matrix

被引:511
作者
Branda, SS
Chu, F
Kearns, DB
Losick, R
Kolter, R [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
[2] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
关键词
D O I
10.1111/j.1365-2958.2005.05020.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microbes construct structurally complex multicellular communities (biofilms) through production of an extracellular matrix. Here we present evidence from scanning electron microscopy showing that a wild strain of the Gram positive bacterium Bacillus subtilis builds such a matrix. Genetic, biochemical and cytological evidence indicates that the matrix is composed predominantly of a protein component, TasA, and an exopolysaccharide component. The absence of TasA or the exopolysaccharide resulted in a residual matrix, while the absence of both components led to complete failure to form complex multicellular communities. Extracellular complementation experiments revealed that a functional matrix can be assembled even when TasA and the exopolysaccharide are produced by different cells, reinforcing the view that the components contribute to matrix formation in an extracellular manner. Having defined the major components of the biofilm matrix and the control of their synthesis by the global regulator SinR, we present a working model for how B. subtilis switches between nomadic and sedentary lifestyles.
引用
收藏
页码:1229 / 1238
页数:10
相关论文
共 31 条
  • [11] Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis
    Hamon, MA
    Stanley, NR
    Britton, RA
    Grossman, AD
    Lazazzera, BA
    [J]. MOLECULAR MICROBIOLOGY, 2004, 52 (03) : 847 - 860
  • [12] The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
    Hamon, MA
    Lazazzera, BA
    [J]. MOLECULAR MICROBIOLOGY, 2001, 42 (05) : 1199 - 1209
  • [13] Harwood CR., 1990, MOL BIOL METHODS BAC
  • [14] Proteome analysis of Bacillus subtilis extracellular proteins:: a two-dimensional protein electrophoretic study
    Hirose, I
    Sano, K
    Shioda, I
    Kumano, M
    Nakamura, K
    Yamane, K
    [J]. MICROBIOLOGY-SGM, 2000, 146 : 65 - 75
  • [15] A master regulator for biofilm formation by Bacillus subtilis
    Kearns, DB
    Chu, F
    Branda, SS
    Kolter, R
    Losick, R
    [J]. MOLECULAR MICROBIOLOGY, 2005, 55 (03) : 739 - 749
  • [16] HIGH-LEVEL TRANSCRIPTION OF THE MAJOR BACILLUS-SUBTILIS AUTOLYSIN OPERON DEPENDS ON EXPRESSION OF THE SIGMA D-GENE AND IS AFFECTED BY A SIN (FLAD) MUTATION
    KURODA, A
    SEKIGUCHI, J
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (03) : 795 - 801
  • [17] Bacillus subtilis α-phosphoglucomutase is required for normal cell morphology and biofilm formation
    Lazarevic, V
    Soldo, B
    Médico, N
    Pooley, H
    Bron, S
    Karamata, D
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (01) : 39 - 45
  • [18] MARQUEZMAGANA LM, 1994, J BACTERIOL, V176, P2435
  • [19] O'Toole GA, 1999, METHOD ENZYMOL, V310, P91
  • [20] Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways:: a genetic analysis
    O'Toole, GA
    Kolter, R
    [J]. MOLECULAR MICROBIOLOGY, 1998, 28 (03) : 449 - 461