Identification of two processed pseudogenes of the human Tom20 gene

被引:3
作者
Hernández, JM [1 ]
Blat, B [1 ]
Iruela, C [1 ]
Vila, F [1 ]
Hernández-Yago, J [1 ]
机构
[1] Fdn Valenciana Invest Biomed, Inst Invest Citol, E-46010 Valencia, Spain
来源
MOLECULAR AND GENERAL GENETICS | 1998年 / 258卷 / 1-2期
关键词
mitochondria; import receptor; human Tom20; nucleotide sequence; processed pseudogenes;
D O I
10.1007/s004380050713
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The open reading frame (ORF) of the human Tom20 gene (hTom20) was amplified by PCR from a HeLa cDNA library using primers based on the sequence of HUMRSC145 and cloned into a pET15b vector. Amplification of human genomic DNA using these primers yielded a DNA fragment of the same size as that of the ORF of hTom20 cDNA. Sequencing of this fragment revealed that: (1) it has the same number of base pairs as the ORF of hTom20 cDNA (438 bp); and (2) the two sequences differ by 14 single base pair substitutions (97% similarity) causing eight changes in the amino acid sequence and two premature stop codons. Further amplification of human genomic DNA adaptor-ligated libraries using primers based on HUMRSC145 revealed three different sequence-related genomic regions; one corresponding to the fragment referred above, another corresponding to the hTom20 gene, and a third fragment of which the sequence differs from the ORF of hTom20 cDNA by only 22 base pair substitutions and a deletion of 4 bp. We conclude that, in addition to the hTom20 gene, there are two genomic DNA sequences (Psi 1Tom20 and Psi 2Tom20) that are processed pseudogenes of hTom20. Aspects concerning their evolutionary origin are discussed.
引用
收藏
页码:117 / 122
页数:6
相关论文
共 30 条
[1]   FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].
BONNER, WM ;
LASKEY, RA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88
[2]   INTRONLESS HUMAN DIHYDROFOLATE-REDUCTASE GENES ARE DERIVED FROM PROCESSED RNA MOLECULES [J].
CHEN, MJ ;
SHIMADA, T ;
MOULTON, AD ;
HARRISON, M ;
NIENHUIS, AW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (23) :7435-7439
[4]   PATTERNS OF NUCLEOTIDE SUBSTITUTION IN PSEUDOGENES AND FUNCTIONAL GENES [J].
GOJOBORI, T ;
LI, WH ;
GRAUR, D .
JOURNAL OF MOLECULAR EVOLUTION, 1982, 18 (05) :360-369
[5]   ROLE OF POLYAMINES IN THE TRANSPORT INVITRO OF THE PRECURSOR OF ORNITHINE TRANSCARBAMYLASE [J].
GONZALEZBOSCH, C ;
MARCOTE, MJ ;
HERNANDEZYAGO, J .
BIOCHEMICAL JOURNAL, 1991, 279 :815-820
[6]  
GOPING IS, 1995, FEBS LETT, V373, P45
[7]   A receptor for the import of proteins into human mitochondria [J].
Hanson, B ;
Nuttall, S ;
Hoogenraad, N .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 235 (03) :750-753
[8]   A CRUCIAL ROLE OF THE MITOCHONDRIAL PROTEIN IMPORT RECEPTOR MOM19 FOR THE BIOGENESIS OF MITOCHONDRIA [J].
HARKNESS, TAA ;
NARGANG, FE ;
VANDERKLEI, I ;
NEUPERT, W ;
LILL, R .
JOURNAL OF CELL BIOLOGY, 1994, 124 (05) :637-648
[9]   DUPLICATION-TARGETED DNA METHYLATION AND MUTAGENESIS IN THE EVOLUTION OF EUKARYOTIC CHROMOSOMES [J].
KRICKER, MC ;
DRAKE, JW ;
RADMAN, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (03) :1075-1079
[10]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+