Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants

被引:26
作者
Ariyadasa, Ruvini [1 ]
Stein, Nils [1 ]
机构
[1] Leibniz Inst Plant Genet & Crop Plant Res IPK, D-06466 Gatersleben, Germany
来源
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2012年
关键词
ARTIFICIAL CHROMOSOME LIBRARY; STRUCTURAL VARIATION; FOSMID LIBRARY; GENETIC-MAP; BRACHYPODIUM-DISTACHYON; CUCUMIS-SATIVUS; COMPLEX GENOMES; CONSTRUCTION; SEQUENCE; RICE;
D O I
10.1155/2012/184854
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In the advent of next-generation sequencing (NGS) platforms, map-based sequencing strategy has been recently suppressed being too expensive and laborious. The detailed studies on NGS drafts alone indicated these assemblies remain far from gold standard reference quality, especially when applied on complex genomes. In this context the conventional BAC-based physical mapping has been identified as an important intermediate layer in current hybrid sequencing strategy. BAC-based physical map construction and its integration with high-density genetic maps have benefited from NGS and high-throughput array platforms. This paper addresses the current advancements of BAC-based physical mapping and high-throughput map integration strategies to obtain densely anchored well-ordered physical maps. The resulted maps are of immediate utility while providing a template to harness the maximum benefits of the current NGS platforms.
引用
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页数:11
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