Protein engineering for bioenergy and biomass-based chemicals

被引：14

作者：

Clarke, Neil D. ^{[1
]}

机构：

[1] Genome Inst Singapore, Singapore 138672, Singapore

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2010年 / 20卷 / 04期

关键词：

SITE-DIRECTED MUTAGENESIS; CIRCULAR PERMUTATION; LIPASE-B; BIOSYNTHESIS; EXPLORATION; METABOLISM; EVOLUTION; BIOLOGY; FUELS;

D O I：

10.1016/j.sbi.2010.06.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of biomass to fuels and chemicals faces substantial challenges if it is to be done on a large scale, in a sustainable manner, and at reasonable cost. Some of these challenges can be addressed through protein engineering technologies, for example by improving biomass-processing enzymes or by manipulating the activities and substrate specificity of enzymes in metabolic pathways. Recent highlights include structure-guided chimera design to improve the properties of cellulases, the engineering of synthetic scaffold proteins to enhance metabolic flux, and the broadening of substrate specificity to co-opt metabolic pathways to the production of long-chain branched alcohols.

引用

收藏

页码：527 / 532

页数：6

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