The AFM as a tool for chromosomal dissection - the influence of physical parameters

被引：59

作者：

Stark, RW

Thalhammer, S

Wienberg, J

Heckl, WM

机构：

[1] Univ Munich, Inst Kristallog & Angew Mineral, D-80333 Munich, Germany

[2] Univ Cambridge, Dept Pathol, Cambridge, England

来源：

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING | 1998年 / 66卷 / Suppl 1期

关键词：

D O I：

10.1007/s003390051205

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Human metaphase chromosomes were dissected using an atomic force microscope (AFM) in ambient conditions and in buffer. Cutting with z-modulation in air yielded precise cuts at loading forces F > 17 mu N with a full width at maximum depth of 90 nm. After dissection, the chromosomal material adhered to the tip to be used for further biochemical processing. In liquids, we measured the effects of different types of buffer solution on swelling of the chromosomes and their elastic behaviour.

引用

页码：S579 / S584

页数：6

共 24 条

[1] ATOMIC-FORCE MICROSCOPY OF MAMMALIAN SPERM CHROMATIN [J].