Variable gene segment-specific N-insertions at the signal joint of T-cell receptor Vβ-Dβ recombinations

被引：6

作者：

Kanari, Y ^{[1
]}

Nakagawa, R ^{[1
]}

Arakawa, H ^{[1
]}

Yamagishi, H ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Biophys, Sakyo Ku, Kyoto 60601, Japan

来源：

IMMUNOLOGY LETTERS | 1998年 / 61卷 / 2-3期

关键词：

T-cell receptor beta; V-DJ recombination; signal joint; N-insertion;

D O I：

10.1016/S0165-2478(98)00012-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the V beta-D beta recombinations. N-insertions were detected at signal joints of V beta 2, V beta 3, V beta 10, V beta 18 and V beta 14 but not in V beta 8 and V beta 7. These data show that the N-insertion at signal joints is dependent on the V beta locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 155

页数：5

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