Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2′-bipyridyl) ruthenium derivative tags

被引：55

作者：

Li, Yan ^{[1
]}

Qi, Honglan ^{[1
]}

Fang, Fang ^{[1
]}

Zhang, Chengxiao ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China

来源：

TALANTA | 2007年 / 72卷 / 05期

基金：

中国国家自然科学基金;

关键词：

DNA hybridization; electrogenerated chemiluminescence; amplification; carbon nanotubes; tris(2,2 '-bipyridyl) ruthenium derivatives;

D O I：

10.1016/j.talanta.2007.01.062

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ultrasensitive electrogenerated chemilummescence (ECL) detection method of DNA hybridization based-on single-walled carbon-nanotubes (SWNT) carrying a large number of ruthenium complex tags was developed. The probe single strand DNA (ss-DNA) and ruthenium complex were loaded at SWNT, which was taken as an ECL probe. When the capture ss-DNA with a thiol group was self-assembled onto the surface of gold electrode, and then hybridized with target ss-DNA and further hybridized with the ECL probe to form DNA sandwich conjugate, a strong ECL response was electrochemically generated. The ECL intensity was linearly related to the concentration of perfect-matched target ss-DNA in the range from 2.4 x 10(-14) to 1.7 x 10(-12) M with a detection limit of 9.0 x 10(-15) M. The ECL signal difference permitted to discriminate the perfect-matched target ss-DNA and two-base-mismatched ss-DNA. This work demonstrates that SWNT can provide an amplification platform for carrying a large number of ECL probe and thus resulting in an ultrasensitive ECL detection of DNA hybridization. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：1704 / 1709

页数：6

共 37 条

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