CNP/GC-B system: A new regulator of adipogenesis

被引:21
作者
Katafuchi, Takeshi [1 ]
Garbers, David L. [1 ]
Albanesi, Joseph P. [1 ]
机构
[1] Univ Texas SW Med Ctr Dallas, Dallas, TX 75390 USA
基金
美国国家卫生研究院;
关键词
C-type natriuretic peptide; Guanylyl cyclase-B; cGmP; Adipogenesis; 3T3-L1; cell; AMP-RESPONSE ELEMENT; TRANSCRIPTIONAL REGULATION; NATRIURETIC PEPTIDE; ADIPOCYTE DIFFERENTIATION; GENE-EXPRESSION; PROMOTES ADIPOGENESIS; CELL-DIFFERENTIATION; 3T3-L1; PREADIPOCYTES; CYCLIC-GMP; IN-VITRO;
D O I
10.1016/j.peptides.2010.06.025
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adipogenesis is regulated by a wide variety of compounds. An adipogenic cocktail containing insulin (INS), dexamethasone (DEX) and 3-isobutyl-1-methyl xanthine (IBMX) is routinely used to induce adipogenesis in 3T3-L1 preadipocytes, but the biochemical actions in adipogenesis of IBMX, a non-specific phosphodiesterase inhibitor, are not completely understood. In this study we show that C-type natriuretic peptide (CNP) is an endogenous adipogenesis regulator which can largely replace the function of IBMX. In 3T3-L1 preadipocytes, CNP potently elevated cGMP production through guanylyl cyclase-B (GC-B). Lipid droplets were evident in these cells upon stimulation with CNP for 12 days in the presence of INS and DEX, and their adiposity, evaluated by Oil Red O, was significantly higher than in cells stimulated with INS and DEX only. Membrane-permeable cGMP analogue also enhanced adiposity when cells were cultured together with INS and DEX, and KT5823, a non-specific cGMP-dependent kinase (cGK) inhibitor, suppressed the stimulatory effect of IBMX on adipogenesis, revealing that IBMX-stimulated adipogenesis is mediated through cGK. The enhancement of adiposity elicited by CNP was accompanied by increased mRNA levels of adipocyte-specific genes including those encoding peroxisome proliferator-activated receptor gamma and glucose transporter 4. Interestingly, the mRNA level of CNP itself was markedly enhanced in 3T3-L1 cells upon stimulation with INS, DEX and IBMX, reaching a maximum at 8 h incubation with the cocktail. These observations suggest that the CNP/GC-B system participates in regulation of adipogenesis, particularly at an early stage in the process. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:1906 / 1911
页数:6
相关论文
共 25 条
[1]   Transcriptional regulation of adipogenesis by KLF4 [J].
Birsoy, Kivanc ;
Chen, Zhu ;
Friedman, Jeffrey .
CELL METABOLISM, 2008, 7 (04) :339-347
[2]   Regulation of preadipocyte factor-1 gene expression during 3T3-L1 cell differentiation [J].
Boney, CM ;
Fiedorek, FT ;
Paul, SR ;
Gruppuso, PA .
ENDOCRINOLOGY, 1996, 137 (07) :2923-2928
[3]   The extracellular signal-regulated kinase isoform ERK1 is specifically required for in vitro and in vivo adipogenesis [J].
Bost, F ;
Aouadi, M ;
Caron, L ;
Even, P ;
Belmonte, N ;
Prot, M ;
Dani, C ;
Hofman, P ;
Pagès, G ;
Pouysségur, J ;
Le Marchand-Brustel, Y ;
Binétruy, B .
DIABETES, 2005, 54 (02) :402-411
[4]   Synergism between calcium and cyclic GMP in cyclic AMP response element-dependent transcriptional regulation requires cooperation between CREB and C/EBP-β [J].
Chen, YC ;
Zhuang, SH ;
Cassenaer, S ;
Casteel, DE ;
Gudi, T ;
Boss, GR ;
Pilz, RB .
MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (12) :4066-4082
[5]   Reciprocal antagonism coordinates C-type natriuretic peptide and mitogen-signaling pathways in fibroblasts [J].
Chrisman, TD ;
Garbers, DL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (07) :4293-4299
[6]  
CHRISMAN TD, 1993, J BIOL CHEM, V268, P3698
[7]   Modern science versus the stigma of obesity [J].
Friedman, JM .
NATURE MEDICINE, 2004, 10 (06) :563-569
[8]   Activation of protein kinase B and induction of adipogenesis by insulin in 3T3-L1 preadipocytes - Contribution of phosphoinositide-3,4,5-trisphosphate versus phosphoinositide-3,4-bisphosphate [J].
Gagnon, A ;
Chen, CS ;
Sorisky, A .
DIABETES, 1999, 48 (04) :691-698
[9]   Membrane guanylyl cyclase receptors: an update [J].
Garbers, David L. ;
Chrisman, Ted D. ;
Wiegn, Phi ;
Katafuchi, Takeshi ;
Albanesi, Joseph P. ;
Bielinski, Vincent ;
Barylko, Barbara ;
Redfield, Margaret M. ;
Burnett, John C., Jr. .
TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2006, 17 (06) :251-258
[10]   Protein Kinase G Controls Brown Fat Cell Differentiation and Mitochondrial Biogenesis [J].
Haas, Bodo ;
Mayer, Peter ;
Jennissen, Katja ;
Scholz, Daniela ;
Diaz, Mauricio Berriel ;
Bloch, Wilhelm ;
Herzig, Stephan ;
Faessler, Reinhard ;
Pfeifer, Alexander .
SCIENCE SIGNALING, 2009, 2 (99) :ra78