Glutamine increases argininosuccinate synthetase mRNA levels in rat hepatocytes - The involvement of cell swelling

被引：34

作者：

Quillard, M

Husson, A

Lavoinne, A

机构：

[1] FAC MED PHARM ROUEN, GRP BIOCHIM & PHYSIOPATHOL DIGEST & NUTR, F-76803 ST ETIENNE, FRANCE

[2] FAC MED PHARM ROUEN, INST FEDERAT RECH MULTIDISCIPLINAIRES PEPTIDES 23, F-76803 ST ETIENNE, FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 236卷 / 01期

关键词：

cultured hepatocytes; mRNA; argininosuccinate synthetase; glutamine; osmotic stress;

D O I：

10.1111/j.1432-1033.1996.00056.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutamine, one of the most efficient substrates in the urea cycle, was found to induce the accumulation of argininosuccinate synthetase (ASS) mRNA in primary cultured rat hepatocytes. The inducing action of glutamine was obtained at various stages of development and a half-maximal effect was observed at about 3 mM glutamine. This effect was apparent from 6 h and persisted for at least 48 h. NH4Cl addition up to 5 mM did not significantly change the ASS mRNA level. Like glutamine, hypoosmotic medium and aminoisobutyric acid (conditions known to increase cell volume) also increased the ASS mRNA level, which was, in contrast, decreased by hyperosmotic medium. These results demonstrate that the glutamine-induced swelling may participate in the observed increase of the ASS mRNA level. This increase in the ASS mRNA level was associated with an increase in ASS activity.

引用

页码：56 / 59

页数：4

共 28 条

[11] HORMONAL-REGULATION OF 2 UREA-CYCLE ENZYMES IN CULTURED FETAL HEPATOCYTES [J].