Proteolipidic Composition of Exosomes Changes during Reticulocyte Maturation

被引：167

作者：

Carayon, Kevin ^{[1
,2
]}

Chaoui, Karima ^{[1
,2
]}

Ronzier, Elsa ^{[1
,2
]}

Lazar, Ikrame ^{[1
,2
]}

Bertrand-Michel, Justine ^{[3
]}

Roques, Veronique ^{[3
]}

Balor, Stephanie ^{[4
]}

Terce, Francois ^{[3
]}

Lopez, Andre ^{[1
,2
]}

Salome, Laurence ^{[1
,2
]}

Joly, Etienne ^{[2
]}

机构：

[1] CNRS, Inst Pharmacol & Struct Biol, F-31077 Toulouse, France

[2] Univ Toulouse 3, Univ Toulouse, F-31077 Toulouse, France

[3] CHU Purpan, INSERM CPTP U563, F-31024 Toulouse 3, France

[4] CNRS, Inst Explorat Fonct Genomes, IFR109, Batiment Inst Biol Cellulaire & Genet, F-31062 Toulouse, France

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 39期

关键词：

CELL-DERIVED EXOSOMES; IN-VITRO; MEMBRANE MICRODOMAINS; TRANSFERRIN RECEPTOR; LIPIDS; VESICLES; PHOSPHOLIPIDS; BIOGENESIS; PROTEOMICS; PROTEINS;

D O I：

10.1074/jbc.M111.257444

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

During the orchestrated process leading to mature erythrocytes, reticulocytes must synthesize large amounts of hemoglobin, while eliminating numerous cellular components. Exosomes are small secreted vesicles that play an important role in this process of specific elimination. To understand the mechanisms of proteolipidic sorting leading to their biogenesis, we have explored changes in the composition of exosomes released by reticulocytes during their differentiation, in parallel to their physical properties. By combining proteomic and lipidomic approaches, we found dramatic alterations in the composition of the exosomes retrieved over the course of a 7-day in vitro differentiation protocol. Our data support a previously proposed model, whereby in reticulocytes the biogenesis of exosomes involves several distinct mechanisms for the preferential recruitment of particular proteins and lipids and suggest that the respective prominence of those pathways changes over the course of the differentiation process.

引用

页码：34426 / 34439

页数：14

共 61 条

[1]

Amigorena S, 2000, MEDICINA-BUENOS AIRE, V60, P51

[2]

THE INVOLVEMENT OF THE CA-DEPENDENT K-CHANNEL AND OF THE KCL COTRANSPORT IN SICKLE-CELL DEHYDRATION DURING CYCLIC DEOXYGENATION [J].

APOVO, M ;

BEUZARD, Y ;

GALACTEROS, F ;

BACHIR, D ;

GIRAUD, F .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1994, 1225 (03) :255-258

[3]

An outlook on organization of lipids in membranes: Searching for a realistic connection with the organization of biological membranes [J].

Bagatolli, Luis A. ;

Ipsen, John H. ;

Simonsen, Adam C. ;

Mouritsen, Ole G. .

PROGRESS IN LIPID RESEARCH, 2010, 49 (04) :378-389

[4]

BARRANS A, 1994, J BIOL CHEM, V269, P11572

[5]

Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages [J].

Barres, Celine ;

Blanc, Lionel ;

Bette-Bobillo, Pascale ;

Andre, Sabine ;

Mamoun, Robert ;

Gabius, Hans-Joachim ;

Vidal, Michel .

BLOOD, 2010, 115 (03) :696-705

[6]

Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins [J].

Bickel, PE ;

Scherer, PE ;

Schnitzer, JE ;

Oh, P ;

Lisanti, MP ;

Lodish, HF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (21) :13793-13802

[7]

Reticulocyte membrane remodeling: contribution of the exosome pathway [J].

Blanc, Lionel ;

Vidal, Michel .

CURRENT OPINION IN HEMATOLOGY, 2010, 17 (03) :177-183

[8]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[9]

Mascot file parsing and quantification (MFPaQ), a new software to parse, validate, and quantify proteomics data generated by ICAT and SILAC mass spectrometric analyses [J].

Bouyssie, David ;

de Peredo, Anne Gonzalez ;

Mouton, Emmanuelle ;

Albigot, Renaud ;

Roussel, Lucie ;

Ortega, Nathalie ;

Cayrol, Corinne ;

Burlet-Schiltz, Odile ;

Girard, Jean-Philippe ;

Monsarrat, Bernard .

MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (09) :1621-1637

[10]

The α4β1 integrin in sickle cell disease [J].

Brittain, J. E. ;

Parise, L. V. .

TRANSFUSION CLINIQUE ET BIOLOGIQUE, 2008, 15 (1-2) :19-22

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