Making RISC

被引：403

作者：

Kawamata, Tomoko ^{[1
,2
]}

Tomari, Yukihide ^{[1
,2
,3
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

[2] Univ Tokyo, Dept Med Genome Sci, Bunkyo Ku, Tokyo 1130032, Japan

[3] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2010年 / 35卷 / 07期

关键词：

GUIDE-STRAND; RNA CLEAVAGE; MICRORNA MATURATION; ARGONAUTE COMPLEXES; SILENCING COMPLEX; PASSENGER-STRAND; DISTINCT ROLES; SIRNA; SPECIFICITY; RECOGNITION;

D O I：

10.1016/j.tibs.2010.03.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is well established that 20- to 30-nt small RNAs, including small interfering RNAs, microRNAs and Piwi-interacting RNAs, play crucial roles in regulating gene expression and control a surprisingly diverse array of biological processes. These small RNAs cannot work alone: they must form effector ribonucleoprotein complexes RNA-induced silencing complexes (RISCs) - to exert their function. Thus, RISC assembly is a key process in small RNA-mediated silencing. Recent biochemical analyses of RISC assembly, together with new structural studies of Argonaute, the core protein component of RISC, suggest a revised view of how mature RISC, which contains single-stranded guide RNA, is built from small RNAs that are born double-stranded.

引用

页码：368 / 376

页数：9

共 74 条

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