Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：118

作者：

Camara, Johanna E. ^{[1
]}

Hays, Faith A. ^{[1
]}

机构：

[1] Natl Inst Stand & Technol, Div Analyt Chem, Gaithersburg, MD 20899 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 05期

关键词：

antibiotic resistance; beta-Lactamase; Escherichia coli; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;

D O I：

10.1007/s00216-007-1558-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z approximate to 29,000 peak, which was confirmed as beta-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus-species identification.

引用

页码：1633 / 1638

页数：6

共 25 条

[1] Top-down proteomics for rapid identification of intact microorganisms
Demirev, PA
Feldman, AB
Kowalski, P
Lin, JS
[J]. ANALYTICAL CHEMISTRY, 2005, 77 (22) : 7455 - 7461
[2] Rapid detection of antimicrobial-resistant organism carriage: an unmet clinical need
Diekema, DJ
Dodgson, KJ
Sigurdardottir, B
Pfaller, MA
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (07) : 2879 - 2883
[3] Identification of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry
Du, ZM
Yang, RF
Guo, ZB
Song, YJ
Wang, J
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (21) : 5487 - 5491
[4] Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry
Edwards-Jones, V
Claydon, MA
Evason, DJ
Walker, J
Fox, AJ
Gordon, DB
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2000, 49 (03) : 295 - 300
[5] Characterization of intact microorganisms by MALDI mass spectrometry
Fenselau, C
Demirev, PA
[J]. MASS SPECTROMETRY REVIEWS, 2001, 20 (04) : 157 - 171
[6] Molecular detection of antimicrobial resistance
Fluit, AC
Visser, MR
Schmitz, FJ
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (04) : 836 - 871
[7] Optimisation of intact cell MALDI method for fingerprinting of methicillin-resistant Staphylococcus aureus
Jackson, KA
Edwards-Jones, V
Sutton, CW
Fox, AJ
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 62 (03) : 273 - 284
[8] An algorithm for automated bacterial identification using matrix-assisted laser desorption/ionization mass spectrometry
Jarman, KH
Cebula, ST
Saenz, AJ
Petersen, CE
Valentine, NB
Kingsley, MT
Wahl, KL
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (06) : 1217 - 1223
[9] MALDI-TOF mass spectrometry and bacterial taxonomy
Lay, JO
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2000, 19 (08) : 507 - 516
[10] Lay JO, 2001, MASS SPECTROM REV, V20, P172, DOI 10.1002/mas.10003.abs

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