Expression of CD3-ζ on T-cells in primary cervical carcinoma and in metastasis-positive and -negative pelvic lymph nodes

被引:26
作者
de Gruijl, TD
Bontkes, HJ
Peccatori, F
Gallee, MPW
Helmerhorst, TJM
Verheijen, RHM
Aarbiou, J
Mulder, WMC
Walboomers, JMM
Meijer, CJLM
van de Vange, N
Scheper, RJ
机构
[1] Free Univ Amsterdam Hosp, Dept Pathol, European Canc Ctr, Amsterdam Gynaecol Oncol Grp, NL-1007 MB Amsterdam, Netherlands
[2] Free Univ Amsterdam Hosp, Dept Obstet & Gynaecol, NL-1007 MB Amsterdam, Netherlands
[3] Antoni Van Leeuwenhoek Hosp, Dept Pathol, NL-1066 CX Amsterdam, Netherlands
[4] Univ Amsterdam, Acad Med Ctr, Dept Obstet & Gynaecol, NL-1105 AZ Amsterdam, Netherlands
[5] European Inst Oncol, Dept Med Oncol, I-20141 Milan, Italy
关键词
cervical carcinoma; cervical dysplasia; T-lymphocytes; zeta chain;
D O I
10.1038/sj.bjc.6690179
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Lymphocytic infiltrate is often present in cervical cancer lesions, possibly reflecting an ongoing, but ineffective, immune response to the tumour. Recently, evidence has accumulated for systemically impaired T-cell functions in cancer patients, associated with decreased expression of signal-transducing zeta (zeta) chain dimer molecules on circulating T-cells and NK-cells. Here, we report on the intralesional downregulation of zeta chain expression on T-cells in cervical carcinoma. Paraffin-embedded or snap-frozen sections from 24 different cervical cancer specimens were studied. Paraffin-embedded tumour-positive (n = 7) and tumour-negative (n = 15) pelvic lymph nodes were also included in the study. Immunostaining was performed on consecutive sections with antibodies specific for CD3-epsilon or the CD3-associated zeta chain dimer. Antigen retrieval by sodium citrate/microwave treatment was essential for zeta staining of paraffin sections. The amount of zeta positive cells was quantitated and related to the number of CD3-epsilon+ cells in corresponding tumour areas. Of the 24 cervical cancer specimens studied, zeta chain dimer expression was reduced in seven cases and strongly reduced in the other 17 samples. In tonsil control sections, CD3-epsilon and CD3-zeta were always co-expressed in almost equal numbers. Also, both tumour-negative and -positive lymph nodes showed zeta chain expression which equalled that of CD3-epsilon expression. These data indicate that a decreased expression of signal-transducing zeta molecules on tumour-infiltrating T-cells is frequent in cervical cancer. The apparently unimpaired zeta chain expression within draining lymph nodes suggests that local tumour-derived factors at the primary site are instrumental in zeta chain down-regulation.
引用
收藏
页码:1127 / 1132
页数:6
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