Neuroprotective effect of nicardipine on cadmium-induced Purkinje cell death in rat, cerebellum

被引:11
作者
Bagirici, F [1 ]
Genç, H
Tan, F
Demír, S
机构
[1] Ondokuz Mayis Univ, Fac Med, Dept Physiol, TR-55139 SAMSUN, Turkey
[2] Ondokuz Mayis Univ, Fac Med, Dept Biol, TR-55139 SAMSUN, Turkey
关键词
cadmium; cell death; nicardipine; cerebellum; rat;
D O I
10.1002/nrc.1031
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The present study was designed to investigate the effects of nicardipine on cadmium-induced Purkinje cell loss in cerebellum of rats. Animals were divided into three groups as control, cadmium and cadmium + nicardipine groups. Saline, cadmium sulphate (0,0021 mg/kg) and cadmium sulphate (0,0021 mg/kg) + nicardipine (1 mg/kg) were intracortically administered respectively. All rats were observed for one week, but only the rats in the third group were injected nicardipine (10 mg/kg/day, i.p.) during this period. One week later, all rats were perfused intracardially. Series of frontal sections from cerebella were stained with Thionin staining. Purkinje cells were counted under x400 magnification via a light microscope. Cadmium administration caused 25-35 % decrease in Purkinje cell density in Crus I, Crus II and Lobulus Simplex areas of cerebellum in cadmium group (p <0.05). The Purkinje cell density was 16-23 % less in nicardipine-treated group than control and cells were partially protected from toxic effects of cadmium significantly (p <0.05). These results suggest that cadmium has neurotoxic; effects on Purkinje cells in cerebellum and nicardipine may protect neurons from cadmium-induced toxicity (p <0.05).
引用
收藏
页码:99 / 105
页数:7
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