Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones

被引：3

作者：

Guan, Yuan ^{[1
]}

Chen, Qi ^{[2
]}

Pan, Junsong ^{[1
]}

Li, Zheng ^{[1
]}

He, Huanle ^{[1
]}

Wu, Aizhong ^{[1
]}

Song, Rentao ^{[2
]}

Cai, Run ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

[2] Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China

来源：

PROGRESS IN NATURAL SCIENCE | 2008年 / 18卷 / 02期

基金：

中国国家自然科学基金;

关键词：

cucumber; BAC library; molecular markers; PCR screening; specific clones;

D O I：

10.1016/j.pnsc.2007.10.001

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94, derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes, 15 sequence-characterized amplified regions (SCAR) and seven simple sequence repeats (SSR) markers from each linkage group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen markers gave at least two positive clones. As a result, 22 BAC clones representing 7 linkage groups of cucumber were identified, which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.

引用

页码：143 / 147

页数：5

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