The vertebrate Hef ortholog is a component of the Fanconi anemia tumor-suppressor pathway

被引：157

作者：

Mosedale, G

Niedzwiedz, W

Alpi, A

Perrina, F

Pereira-Leal, JB

Johnson, M

Langevin, F

Pace, P

Patel, KJ

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Addenbrookes Hosp, Dept Med, Cambridge CB2 2QH, England

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 09期

关键词：

D O I：

10.1038/nsmb981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The helicase-associated endonuclease for fork-structured DNA (Hef) is an archaeabacterial protein that processes blocked replication forks. Here we have isolated the vertebrate Hef ortholog and investigated its molecular function. Disruption of this gene in chicken DT40 cells results in genomic instability and sensitivity to DNA cross-links. The similarity of this phenotype to that of cells lacking the Fanconi anemia - related (FA) tumor-suppressor genes led us to investigate whether Hef functions in this pathway. Indeed, we found a genetic interaction between the FANCC and Hef genes. In addition, Hef is a component of the FA nuclear protein complex that facilitates its DNA damage - inducible chromatin localization and the monoubiquitination of the FA protein FANCD2. Notably, Hef interacts directly with DNA structures that are intermediates in DNA replication. This discovery sheds light on the origins, regulation and molecular function of the FA tumor-suppressor pathway in the maintenance of genome stability.

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页码：763 / 771

页数：9

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