Trapping intermediates in the crystal: ligand binding to myoglobin

被引：59

作者：

Schlichting, I

Chu, K

机构：

[1] Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44227 Dortmund, Germany

[2] Univ Vermont, Dept Phys, Burlington, VT 05405 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2000年 / 10卷 / 06期

关键词：

D O I：

10.1016/S0959-440X(00)00158-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystal structures of the reactive short-lived species that occur in chemical or binding reactions can be determined using X-ray crystallography via time-resolved or kinetic trapping approaches. Recently, various kinetic trapping methods have been used to determine the structure of intermediates in ligand binding to myoglobin.

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页码：744 / 752

页数：9

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