Calcium-sensing receptor attenuates AVP-induced aquaporin-2 expression via a calmodulin-dependent mechanism

被引：76

作者：

Bustamante, Mauro ^{[1
,2
]}

Hasler, Udo ^{[1
]}

Leroy, Valerie ^{[1
]}

de Seigneux, Sophie ^{[1
]}

Dimitrov, Mitko ^{[1
]}

Mordasini, David ^{[1
]}

Rousselot, Martine ^{[1
]}

Martin, Pierre-Yves ^{[1
]}

Feraille, Eric ^{[1
]}

机构：

[1] Fdn Pour Red Med, Serv Nephrol, CH-1211 Geneva, Switzerland

[2] Inst Rech Ophtalmol, Sion, Switzerland

来源：

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | 2008年 / 19卷 / 01期

关键词：

D O I：

10.1681/ASN.2007010092

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Recent evidence suggests that arginine vasopressin (AVP)-dependent aquaporin-2 expression is modulated by the extracellular calcium-sensing receptor (CaSR) in principal cells of the collecting duct, but the signaling pathways mediating this effect are unknown. Using a mouse cortical collecting duct cell line (mpkCCD(cl4)), we found that increasing the concentration of apical extracellular calcium or treating with the CaSR agonists neomycin or Gd3+ attenuated AVP-dependent accumulation of aquaporin-2 mRNA and protein; CaSR gene-silencing prevented this effect. Calcium reduced the AVP-induced accumulation of cAMP, but this did not occur by increased degradation of cAMP by phosphodiesterases or by direct inhibition of adenylate cyclase. Notably, the effect of extracellular calcium on AVP-dependent aquaporin-2 expression was prevented by inhibition of calmodulin. In summary, our results show that high concentrations of extracellular calcium attenuate AVP-incluced aquaporin-2 expression by activating the CaSR and reducing coupling efficiency between V-2 receptor and adenylate cyclase via a calmodulin-dependent mechanism in cultured cortical collecting duct cells.

引用

页码：109 / 116

页数：8

共 23 条

[21] Distribution of aquaporin-4 water channel expression within rat kidney [J].

Terris, J ;

Ecelbarger, CA ;

Marples, D ;

Knepper, MA ;

Nielsen, S .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1995, 269 (06) :F775-F785

[22] Long-term regulation of four renal aquaporins in rats [J].

Terris, J ;

Ecelbarger, CA ;

Nielsen, S ;

Knepper, MA .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1996, 271 (02) :F414-F422

[23] AQP3, p-AQP2, and AQP2 expression is reduced in polyuric rats with hypercalcemia:: prevention by cAMP-PDE inhibitors [J].

Wang, WD ;

Li, CL ;

Kwon, TH ;

Knepper, MA ;

Frokiær, J ;

Nielsen, S .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2002, 283 (06) :F1313-F1325

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