Less label, more free: Approaches in label-free quantitative mass spectrometry

被引：525

作者：

Neilson, Karlie A. ^{[1
]}

Ali, Naveid A. ^{[1
]}

Muralidharan, Sridevi ^{[1
]}

Mirzaei, Mehdi ^{[1
]}

Mariani, Michael ^{[1
]}

Assadourian, Garine ^{[1
]}

Lee, Albert ^{[2
]}

van Sluyter, Steven C. ^{[1
]}

Haynes, Paul A. ^{[1
]}

机构：

[1] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia

[2] NYU, Sch Med, Dept Cardiothorac Surg, New York, NY 10003 USA

来源：

PROTEOMICS | 2011年 / 11卷 / 04期

基金：

澳大利亚研究理事会;

关键词：

Label-free quantitation; NSAF; Quantitative proteomics; Shotgun proteomics; Spectral counting; Technology; SPECTRAL COUNT DATA; LIQUID-CHROMATOGRAPHY; SHOTGUN PROTEOMICS; SACCHAROMYCES-CEREVISIAE; ABSOLUTE QUANTIFICATION; LC-MS/MS; PROTEIN EXPRESSION; DIFFERENTIAL EXPRESSION; RELATIVE QUANTITATION; BIOMARKER DISCOVERY;

D O I：

10.1002/pmic.201000553

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this review we examine techniques, software, and statistical analyses used in label-free quantitative proteomics studies for area under the curve and spectral counting approaches. Recent advances in the field are discussed in an order that reflects a logical workflow design. Examples of studies that follow this design are presented to highlight the requirement for statistical assessment and further experiments to validate results from label-free quantitation. Limitations of label-free approaches are considered, label-free approaches are compared with labelling techniques, and forward-looking applications for label-free quantitative data are presented. We conclude that label-free quantitative proteomics is a reliable, versatile, and cost-effective alternative to labelled quantitation.

引用

页码：535 / 553

页数：19

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