Application of polymerase chain reaction-based assays for rapid identification and antibiotic resistance screening of Helicobacter pylori in gastric biopsies

被引：22

作者：

Chisholm, Stephanie A. ^{[1
]}

Owen, Robert J. ^{[1
]}

机构：

[1] Hlth Protect Agcy, Ctr Infect, Gastrointestinal Emerging & Zoonot Infect Dept, London NW9 5HT, England

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 01期

关键词：

Helicobacter pylori; PCR; gastric biopsy; detection; antibiotic resistance;

D O I：

10.1016/j.diagmicrobio.2007.12.005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The benefits of using a multiplex detection polymerase chain reaction (PCR) assay for Helicobacter pylori speciation and 2 real-time probe hybridization assays determining clarithromycin and tetracycline susceptibilities in gastric biopsies from 171 dyspeptic patients were investigated. Overall, 70 of 71 H. pylori culture-positive biopsies were PCR positive. For the 100 culture-negative biopsies, PCR identified a further 29 H. pylori positives (17% overall) and presence of resistance markers for clarithromycin (20/28) and tetracycline (2/28). The results demonstrated that PCR testing was valuable in providing improved detection rates and antibiotic susceptibility information when H. pylori culture was unsuccessful. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：67 / 71

页数：5

共 28 条

[1]

Björkholm B, 1998, J CLIN MICROBIOL, V36, P3689

[2]

Can F, 2005, CAN J MICROBIOL, V51, P569, DOI [10.1139/w05-035, 10.1139/W05-035]

[3] Development and application of a novel screening PCR assay for direct detection of 'Helicobacter heilmannii'-like organisms in human gastric biopsies in Southeast England [J].

Chisholm, SA ;

Owen, RJ .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2003, 46 (01) :1-7

[4] PCR-Based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples [J].

Chisholm, SA ;

Owen, RJ ;

Teare, EL ;

Saverymuttu, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) :1217-1220

[5] Effect of pretreatment antibiotic resistance to metronidazole and clarithromycin on outcome of Helicobacter pylori therapy -: A meta-analytical approach [J].

Dore, MP ;

Leandro, G ;

Realdi, G ;

Sepulveda, AR ;

Graham, DY .

DIGESTIVE DISEASES AND SCIENCES, 2000, 45 (01) :68-76

[6] Helicobacter pylori antibiotic resistance patterns and genotypes in adult dyspeptic patients from a regional population in North Wales [J].

Elviss, NC ;

Owen, RJ ;

Xerry, J ;

Walker, AM ;

Davies, K .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (02) :435-440

[7] New site of modification of 23S rRNA associated with clarithromycin resistance of Helicobacter pylori clinical isolates [J].

Fontana, C ;

Favaro, M ;

Minelli, S ;

Criscuolo, AA ;

Pietroiusti, A ;

Galante, A ;

Favalli, C .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (12) :3765-3769

[8] 16S rRNA mutation-mediated tetracycline resistance in Helicobacter pylori [J].

Gerrits, MM ;

de Zoete, MR ;

Arents, NLA ;

Kuipers, EJ ;

Kusters, JG .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) :2996-3000

[9] Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori [J].

Gibson, JR ;

Saunders, NA ;

Burke, B ;

Owen, RJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) :3746-3748

[10] Real-time PCR screening for 16S rRNA mutations associated with resistance to tetracycline in Helicobacter pylori [J].

Glocker, E ;

Berning, M ;

Gerrits, MM ;

Kusters, JG ;

Kist, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (08) :3166-3170

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