Application of polymerase chain reaction-based assays for rapid identification and antibiotic resistance screening of Helicobacter pylori in gastric biopsies

被引：22

作者：

Chisholm, Stephanie A. ^{[1
]}

Owen, Robert J. ^{[1
]}

机构：

[1] Hlth Protect Agcy, Ctr Infect, Gastrointestinal Emerging & Zoonot Infect Dept, London NW9 5HT, England

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 01期

关键词：

Helicobacter pylori; PCR; gastric biopsy; detection; antibiotic resistance;

D O I：

10.1016/j.diagmicrobio.2007.12.005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The benefits of using a multiplex detection polymerase chain reaction (PCR) assay for Helicobacter pylori speciation and 2 real-time probe hybridization assays determining clarithromycin and tetracycline susceptibilities in gastric biopsies from 171 dyspeptic patients were investigated. Overall, 70 of 71 H. pylori culture-positive biopsies were PCR positive. For the 100 culture-negative biopsies, PCR identified a further 29 H. pylori positives (17% overall) and presence of resistance markers for clarithromycin (20/28) and tetracycline (2/28). The results demonstrated that PCR testing was valuable in providing improved detection rates and antibiotic susceptibility information when H. pylori culture was unsuccessful. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：67 / 71

页数：5

共 28 条

[21] Simultaneous colonisation of Helicobacter pylori with and without mutations in the 23S rRNA gene in patients with no history of clarithromycin exposure [J].