Chemoenzymatic Synthesis of Acyl Coenzyme A Substrates Enables in Situ Labeling of Small Molecules and Proteins

被引：34

作者：

Agarwal, Vinayak ^{[1
]}

Diethelm, Stefan ^{[2
]}

Ray, Lauren ^{[2
]}

Garg, Neha ^{[3
]}

Awakawa, Takayoshi ^{[2
,4
]}

Dorrestein, Pieter C. ^{[3
]}

Moore, Bradley S. ^{[1
,2
,3
]}

机构：

[1] Univ Calif San Diego, Ctr Oceans & Human Hlth, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Ctr Marine Biotechnol & Biomed, Scripps Inst Oceanog, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[4] Univ Tokyo, Grad Sch Pharmaceut Sci, Tokyo 1130033, Japan

来源：

ORGANIC LETTERS | 2015年 / 17卷 / 18期

基金：

瑞士国家科学基金会;

关键词：

BIOSYNTHESIS; POLYKETIDE; THIOESTERS;

D O I：

10.1021/acs.orglett.5b02113

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A chemoenzymatic approach to generate fully functional acyl coenzyme A molecules that are then used as substrates to drive in situ acyl transfer reactions is described. Mass spectrometry based assays to verify the identity of acyl coenzyme A enzymatic products are also illustrated. The approach is responsive to a diverse array of carboxylic acids that can be elaborated to their corresponding coenzyme A thioesters, with potential applications in wide-ranging chemical biology studies that utilize acyl coenzyme A substrates.

引用

页码：4452 / 4455

页数：4

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