Preparation and in vitro analysis of microencapsulated live Lactobacillus fermentum 11976 for augmentation of feruloyl esterase in the gastrointestinal tract

被引:25
作者
Bhathena, Jasmine [1 ,2 ]
Kulamarva, Arun [1 ,2 ]
Martoni, Christopher [1 ,2 ]
Urbanska, Aleksandra Malgorzata [1 ,2 ]
Prakash, Satya [1 ,2 ]
机构
[1] McGill Univ, Fac Med, Biomed Technol & Cell Therapy Res Lab, Dept Biomed Engn & Artificial Cells, Montreal, PQ H3A 2B4, Canada
[2] McGill Univ, Fac Med, Organs Res Ctr, Montreal, PQ H3A 2B4, Canada
关键词
ferulic acid (FA); feruloyl esterase (FAE); gastrointestinal (GI) tract; Lactobacillus fermentum; microencapsulated bacteria; oral delivery;
D O I
10.1042/BA20070007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
FA (ferulic acid) is a well-known phenolic phytochemical present in plant cell walls. Various studies have indicated that FA has many physiological functions in the prevention of chronic disease. It has been shown to play an important chemoprotective role in degenerative diseases. FA also shows strong antioxidant and nitrite-scavenging potential and anticarcinogenic and anti-inflammatory properties. The in vivo physiological importance of FA depends on its availability for absorption. Dietary fibre-bound FA is partially released by gut micro-organ isms; however, the concentration of the released FA is too low to act as a chemopreventive agent. Therefore it is important to augment the bioavailability of FA to appreciate more fully its real physiological effect. This paper evaluates the suitability of the alginate-poly(L-lysine)-alginate microcapsules for oral delivery of live feruloyl esterase-producing Lactobacillus fermentum 11976 cells, in vitro, by using a dynamic simulated human GI (gastrointestinal) model. The present study shows that microencapsulated L. fermentum 11976 cells can efficiently break down a FA-containing substrate, and establishes the biotechnological basis for their use in supplementing the bioavailability of dietary FA in the intestine.
引用
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页码:1 / 9
页数:9
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