DNA biosensor based on chitosan film doped with carbon nanotubes

被引：158

作者：

Li, J ^{[1
]}

Liu, Q ^{[1
]}

Liu, YJ ^{[1
]}

Liu, SC ^{[1
]}

Yao, SZ ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab ChemoBiosensing & Chemometr, Changsha 410082, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 346卷 / 01期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

chitosan; carbon nanotubes; fish sperm DNA; methylene blue; differential pulse voltammetry;

D O I：

10.1016/j.ab.2005.07.037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A biosensor based on chitosan doped with carbon nanotube (CNT) was fabricated to detect salmon sperm DNA. Methylene blue (MB) was employed as a DNA indicator. It was found that CNTs can enhance the electroactive surface area threefold (0.28 +/- 0.03 and 0.093 +/- 0.06 cm(2) for chitosan-CNT- and chitosan-modified electrodes, respectively) and can accelerate the rate of electron transfer between the redox-active MB and the electrode. A low detection limit of 0.252 nM fish sperm DNA was achieved, and no interference was found in the presence of 5 mu g/ml human serum albumin. The differential pulse voltammetry signal of MB was linear over the fish sperm DNA concentration range of 0.5-20 nM. (C) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：107 / 114

页数：8

相关论文

共 31 条

[1]

Bard A. J., 2000, ELECTROCHEMICAL METH

[2] Carbon nanotube actuators [J].

Baughman, RH ;

Cui, CX ;

Zakhidov, AA ;

Iqbal, Z ;

Barisci, JN ;

Spinks, GM ;

Wallace, GG ;

Mazzoldi, A ;

De Rossi, D ;

Rinzler, AG ;

Jaschinski, O ;

Roth, S ;

Kertesz, M .

SCIENCE, 1999, 284 (5418) :1340-1344

[3] Carbon nanotube films as electron field emitters [J].

Bonard, JM ;

Croci, M ;

Klinke, C ;

Kurt, R ;

Noury, O ;

Weiss, N .

CARBON, 2002, 40 (10) :1715-1728

[4] Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection [J].

Cai, H ;

Cao, XN ;

Jiang, Y ;

He, PG ;

Fang, YZ .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 375 (02) :287-293

[5] Ultrasensitive detection of DNA sequences in solution by specific enzymatic labeling [J].

Castro, A ;

Dalvit, DAR ;

Paz-Matos, L .

ANALYTICAL CHEMISTRY, 2004, 76 (14) :4169-4174

[6] Sol-gel-derived ceramic-carbon nanotube nanocomposite electrodes: Tunable electrode dimension and potential electrochemical applications [J].

Gong, KP ;

Zhang, MN ;

Yan, YM ;

Su, L ;

Mao, LQ ;

Xiong, ZX ;

Chen, Y .

ANALYTICAL CHEMISTRY, 2004, 76 (21) :6500-6505

[7] Characterization and optimization of a real-time, parallel, label-free, polypyrrole-based DNA sensor by surface plasmon resonance imaging [J].

Guedon, P ;

Livache, T ;

Martin, F ;

Lesbre, F ;

Roget, A ;

Bidan, G ;

Levy, Y .

ANALYTICAL CHEMISTRY, 2000, 72 (24) :6003-6009

[8] Electrostatic assembly of calf thymus DNA on multi-walled carbon nanotube modified gold electrode and its interaction with chlorpromazine hydrochloride [J].

Guo, ML ;

Chen, JH ;

Nie, LH ;

Yao, SZ .

ELECTROCHIMICA ACTA, 2004, 49 (16) :2637-2643

[9] Electrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes [J].

Hrapovic, S ;

Liu, YL ;

Male, KB ;

Luong, JHT .

ANALYTICAL CHEMISTRY, 2004, 76 (04) :1083-1088

[10] Electrochemical characterization of cetyltrimethyl ammonium bromide modified carbon paste electrode and the application in the immobilization of DNA [J].

Hu, CG ;

Hu, SS .

ELECTROCHIMICA ACTA, 2004, 49 (03) :405-412

← 1 2 3 4 →