Intracellular localization of lipoplexed siRNA in vascular endothelial cells of different mouse tissues

被引：21

作者：

Aleku, Manuela ^{[1
]}

Fisch, Gerald ^{[1
]}

Moepert, Kristin ^{[1
]}

Keil, Oliver ^{[1
]}

Arnold, Wolfgang ^{[1
]}

Kaufmann, Joerg ^{[1
]}

Santel, Ansgar ^{[1
]}

机构：

[1] Silence Therapeut AG, D-13125 Berlin, Germany

来源：

MICROVASCULAR RESEARCH | 2008年 / 76卷 / 01期

关键词：

RNAi in vivo; liposomal delivery; cationic lipid; PECAM-1; CD34; laminin; lung;

D O I：

10.1016/j.mvr.2008.02.004

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Liposomally formulated siRNA can be used for RNAi applications in vivo. Intravenous bolus administration of lipoplexed siRNA has been shown to reduce gene expression in the vascular endothelium. Here, we applied immunofluorescence staining for different endothelial markers (PECAM-1, CD34, laminin) on paraffin sections to compare the respective expression pattern with the intracellular localization of intravenously administered, fluorescently labeled siRNA (siRNA-Cy3-lipoplex). By confocal microscopy, lipoplexed siRNA-Cy3 was detected inside vascular endothelial cells in vivo, which where identified with co-staining of endothelial markers. Consequently, the finding of intracellular siRNA uptake by vascular endothelial cells correlated with RNAi based specific protein reduction in situ as revealed by PECAM-1 specific immunofluorescence staining in lung tissue sections. Therefore, by using a cell biological approach these in situ data emphasize the functional uptake of liposomal siRNA molecules in vascular endothelial cells of different mouse tissues as indicated in our previous molecular study. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：31 / 41

页数：11

共 35 条

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