The use of mass spectrometry in genomics

被引：25

作者：

Meng, ZJ ^{[1
]}

Simmons-Willis, TA ^{[1
]}

Limbach, PA ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Chem, Rieveschl Labs Mass Spectrometry, Cincinnati, OH 45221 USA

来源：

BIOMOLECULAR ENGINEERING | 2004年 / 21卷 / 01期

基金：

美国国家卫生研究院;

关键词：

electrospray ionization; matrix-assisted laser desorption/ionization; nucleic acids; oligonucleotides; DNA; single nucleotide polymorphism; short tandem repeats; epigenetics; SNP; STR; MALDI; ESI;

D O I：

10.1016/j.bioeng.2003.08.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) now permit the near routine analysis of oligonucleotides and intact nucleic acids. These developments have led to the use of mass spectrometry (MS) as a detection platform for genomics studies. Among the various uses of mass spectrometry in genomics, applications focused on the characterization of single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs) are particularly well-suited to MALDI or ESI-based analysis. It is predicted that continued developments in methodology and instrumentation will further improve the capabilities of mass spectrometry for nucleic acid analysis. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1 / 13

页数：13

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