Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate

被引:75
作者
Mohagheghi, A [1 ]
Dowe, N [1 ]
Schell, D [1 ]
Chou, YC [1 ]
Eddy, C [1 ]
Zhang, M [1 ]
机构
[1] Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA
关键词
corn stover; ethanol; overliming; pretreatment; Zymomonas mobilis;
D O I
10.1023/B:BILE.0000015451.96737.96
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Efficient conversion of lignocellulosic biomass requires biocatalysts able to tolerate inhibitors produced by many pretreatment processes. Recombinant Zymomonas mobilis 8b, a recently developed integrant of Zymomonas mobilis 31821(pZB5), tolerated acetic acid up to 16 g l(-1) and achieved 82%-87% (w/w) ethanol yields from pure glucose/xylose solutions at pH 6 and temperatures of 30degreesC and 37degreesC. An ethanol yield of 85% (w/w) was achieved on glucose/xylose from hydrolysate produced by dilute sulfuric acid pretreatment of corn stover after an 'overliming' process was used to improve hydrolysate fermentability.
引用
收藏
页码:321 / 325
页数:5
相关论文
共 16 条
[1]   Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae [J].
Delgenes, JP ;
Moletta, R ;
Navarro, JM .
ENZYME AND MICROBIAL TECHNOLOGY, 1996, 19 (03) :220-225
[2]   Kinetic analysis of ethanol production by an acetate-resistant strain of recombinant Zymomonas mobilis [J].
Jeon, YJ ;
Svenson, CJ ;
Joachimsthal, EL ;
Rogers, PL .
BIOTECHNOLOGY LETTERS, 2002, 24 (10) :819-824
[3]   A mutant of Zymomonas mobilis ZM4 capable of ethanol production from glucose in the presence of high acetate concentrations. [J].
Joachimstahl, E ;
Haggett, KD ;
Jang, JH ;
Rogers, PL .
BIOTECHNOLOGY LETTERS, 1998, 20 (02) :137-142
[4]  
Kim IM, 2003, J MICROBIOL BIOTECHN, V13, P976
[5]   Nuclear magnetic resonance studies of acetic acid inhibition of rec Zymomonas mobilis ZM4(pZB5) [J].
Kim, IS ;
Barrow, KD ;
Rogers, PL .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2000, 84-6 (1-9) :357-370
[6]   Comparative ethanol productivities of different Zymomonas recombinants fermenting oat hull hydrolysate [J].
Lawford, HG ;
Rousseau, JD ;
Tolan, JS .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2001, 91-3 (1-9) :133-146
[7]   Continuous culture studies of xylose-fermenting Zymomonas mobilis [J].
Lawford, HG ;
Rousseau, JD ;
Mohagheghi, A ;
McMillan, JD .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1998, 70-2 (1) :353-367
[8]  
LAWFORD HG, 1990, BIOTECHNOL APPL BIOC, V12, P206
[9]   THE EFFECT OF ACETIC-ACID ON FUEL ETHANOL-PRODUCTION BY ZYMOMONAS [J].
LAWFORD, HG ;
ROUSSEAU, JD .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 39 :687-699
[10]   THE PH-DEPENDENT ENERGETIC UNCOUPLING OF ZYMOMONAS BY ACETIC-ACID [J].
LAWFORD, HG ;
ROUSSEAU, JD .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1994, 45-6 :437-448