The ergot alkaloid gene cluster in Claviceps purpurea:: Extension of the cluster sequence and intra species evolution

被引：80

作者：

Haarmann, T

Machado, C

Lübbe, Y

Correia, T

Schardl, CL

Panaccione, DG

Tudzynski, P

机构：

[1] Univ Munster, Inst Bot, D-48149 Munster, Germany

[2] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA

[3] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA

来源：

PHYTOCHEMISTRY | 2005年 / 66卷 / 11期

关键词：

Claviceps purpurea; ergot fungus; ergot alkaloids; gene cluster; phosphate effect; nonribosomal peptide synthetases; chemical races; ergotamine; ergocristine;

D O I：

10.1016/j.phytochem.2005.04.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Holter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1312 / 1320

页数：9

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