A nuclear gene encoding mitochondrial △1-pyrroline-5-carboxylate dehydrogenase and its potential role in protection from proline toxicity

被引:170
作者
Deuschle, K
Funck, D
Hellmann, H
Däschner, K
Binder, S
Frommer, WB
机构
[1] ZMBP, D-72076 Tubingen, Germany
[2] Univ Ulm, D-89069 Ulm, Germany
关键词
Arabidopsis; cell death; pathogen response; proline metabolism; transcriptional regulation; yeast;
D O I
10.1046/j.1365-313X.2001.01101.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Delta (1)-pyrroline-5-carboxylate (P5C), an intermediate in biosynthesis and degradation of proline (Pro), is assumed to play a role in cell death in plants and animals. Toxicity of external Pro and P5C supply to Arabidopsis suggested that P5C dehydrogenase (P5CDH; EC 1.5.1.12) plays a crucial role in this process by degrading the toxic Pro catabolism intermediate P5C. Also in a Delta put2 yeast mutant, lacking P5CDH, Pro led to growth inhibition and formation of reactive oxygen species (ROS). Complementation of the Delta put2 mutant allowed identification of the Arabidopsis P5CDH gene. AtP5CDH is a single-copy gene and the encoded protein was localized to the mitochondria. High homology of AtP5CDH to LuFIS1, an mRNA upregulated during susceptible pathogen attack in flax, suggested a role for P5CDH in inhibition of hypersensitive reactions. An Arabidopsis mutant (cpr5) displaying a constitutive pathogen response was found to be hypersensitive to external Pro. In agreement with a role in prevention of cell death, AtP5CDH was expressed at a basal level in all tissues analysed. The highest expression was found in flowers that are known to contain the highest Pro levels under normal conditions. External supply of Pro induced AtP5CDH expression, but much more slowly than Pro dehydrogenase (AtProDH) expression. Uncoupled induction of the AtProDH and AtP5CDH genes further supports the hypothesis that P5C levels have to be tightly controlled. These results indicate that, in addition to the well-studied functions of Pro, for example in osmoregulation, the Pro metabolism intermediate P5C also serves as a regulator of cellular stress responses.
引用
收藏
页码:345 / 355
页数:11
相关论文
共 44 条
[11]   SUB-MITOCHONDRIAL LOCATION AND ELECTRON-TRANSPORT CHARACTERISTICS OF ENZYMES INVOLVED IN PROLINE OXIDATION [J].
ELTHON, TE ;
STEWART, CR .
PLANT PHYSIOLOGY, 1981, 67 (04) :780-784
[12]   Two delta(1)-pyrroline-5-carboxylate dehydrogenase isoforms are expressed in cultured Nicotiana plumbaginifolia cells and are differentially modulated during the culture growth cycle [J].
Forlani, G ;
Scainelli, D ;
Nielsen, E .
PLANTA, 1997, 202 (02) :242-248
[13]   Delta(1)-pyrroline-5-carboxylate dehydrogenase from cultured cells of potato - Purification and properties [J].
Forlani, G ;
Scainelli, D ;
Nielsen, E .
PLANT PHYSIOLOGY, 1997, 113 (04) :1413-1418
[14]   Expression of δ1-pyrroline-5-carboxylate dehydrogenase and proline/arginine homeostasis in Solanum tuberosum [J].
Forlani, G ;
Mangiagalli, A ;
Pinter, C ;
Nielsen, E .
PHYSIOLOGIA PLANTARUM, 2000, 110 (01) :22-27
[15]   UTILIZATION OF EXOGENOUS PYRIMIDINES AND RECYCYCLING OF URIDINE-5'-PHOSPHATE DERIVATIVES IN SACCHAROMYCES-CEREVISIAE, AS STUDIED BY MEANS OF MUTANTS AFFECTED IN PYRIMIDINE UPTAKE AND METABOLISM [J].
GRENSON, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 11 (02) :249-&
[16]   Proline synthesis and degradation: a model system for elucidating stress-related signal transduction [J].
Hare, PD ;
Cress, WA ;
van Staden, J .
JOURNAL OF EXPERIMENTAL BOTANY, 1999, 50 (333) :413-434
[17]   Dissecting the roles of osmolyte accumulation during stress [J].
Hare, PD ;
Cress, WA ;
Van Staden, J .
PLANT CELL AND ENVIRONMENT, 1998, 21 (06) :535-553
[18]   Hypersensitivity of an Arabidopsis sugar signaling mutant toward exogenous proline application [J].
Hellmann, H ;
Funck, D ;
Rentsch, D ;
Frommer, WB .
PLANT PHYSIOLOGY, 2000, 123 (02) :779-790
[19]   Products of proline catabolism can induce osmotically regulated genes in rice [J].
Iyer, S ;
Caplan, A .
PLANT PHYSIOLOGY, 1998, 116 (01) :203-211
[20]   Reactive oxygen intermediates as mediators of programmed cell death in plants and animals [J].
Jabs, T .
BIOCHEMICAL PHARMACOLOGY, 1999, 57 (03) :231-245