Bleach gel: A simple agarose gel for analyzing RNA quality

被引:364
作者
Aranda, Patrick S. [1 ]
LaJoie, Dollie M. [1 ]
Jorcyk, Cheryl L. [1 ]
机构
[1] Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA
关键词
Agarose; Bleach; Denaturing gel; RNA quality; DIAZOBENZYLOXYMETHYL-PAPER; MESSENGER-RNA; VIRUS RNASE; PROTEIN; ELECTROPHORESIS; RIBONUCLEASE; IDENTIFICATION; HYBRIDIZATION;
D O I
10.1002/elps.201100335
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive equipment such as microfluidic electrophoretic devices, and as an alternative to the costly and time-consuming standard formaldehyde gel, RNA quality can be quickly analyzed by adding small amounts of commercial bleach to TAE buffer-based agarose gels prior to electrophoresis. In the presence of low concentrations of bleach, the secondary structure of RNA is denatured and potential contaminating RNases are destroyed. Because of this, the bleach gel is a functional approach that addresses the need for an inexpensive and safe way to evaluate RNA integrity and will improve the ability of researchers to rapidly analyze RNA quality.
引用
收藏
页码:366 / 369
页数:4
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