Molecular biology of Porcine circovirus:: analyses of gene expression and viral replication

被引：181

作者：

Mankertz, A ^{[1
]}

Çaliskan, R ^{[1
]}

Hattermann, K ^{[1
]}

Hillenbrand, B ^{[1
]}

Kurzendoerfer, P ^{[1
]}

Mueller, B ^{[1
]}

Schmitt, C ^{[1
]}

Steinfeldt, T ^{[1
]}

Finsterbusch, T ^{[1
]}

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

来源：

VETERINARY MICROBIOLOGY | 2004年 / 98卷 / 02期

关键词：

Porcine circovirus; replication; gene expression;

D O I：

10.1016/j.vetmic.2003.10.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The rep gene of Porcine circovirus type I directs the synthesis of two proteins. The full-length protein Rep is 312 amino acids in size, the spliced variant Rep' is truncated (168 aa) and exon 2 is frame-shifted. Replication of PCV1 DNA depends on synthesis of both proteins. Rep and Rep' bind in vitro to double-stranded DNA fragments comprising part of the origin of replication of PCV1, but the minimal binding sites of the two proteins are distinct. Rep protein represses the promoter of the rep gene by binding to the two inner hexamers HI and H2. Although Rep' binds to the same sequence, it does not influence Prep. Twelve hours after PCV1 infection, similar amounts of rep and rep' were detected by real-time PCR, but later on, the ratio of the two transcripts varied. Both proteins are co-localised in the nucleus and formation of homo- and heteromeric complexes has been observed. When a replication assay was performed, in which Rep and Rep' protein of PCV1 was used to replicate the origin of PCV1 and PCV2, the rep gene products were found to initiate replication at both origins of replication. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：81 / 88

页数：8

共 20 条

[1] Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus [J].