Plate colonization of Methanococcus maripaludis and Methanosarcina thermophila in a modified canning jar

被引：11

作者：

Apolinario, EA ^{[1
]}

Sowers, KR ^{[1
]}

机构：

[1] UNIV MARYLAND,INST BIOTECHNOL,CTR MARINE BIOTECHNOL,COLUMBUS CTR,BALTIMORE,MD 21202

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 145卷 / 01期

关键词：

methanogen; archaea; methylotroph; hydrogen utilizer; plating; anaerobe jar;

D O I：

10.1016/0378-1097(96)00401-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Conditions have been optimized for colonization of fastidiously anaerobic microorganisms on solidified plating medium incubated in modified canning jars. Two species of methanogenic Archaea, the mesophilic, hydrogen utilizer Methanococcus maripaludis and the moderately thermophilic methylotroph Methanosarcina thermophila, were grown with high efficiency on agar-solidified medium in petri plates. Maximum colony size and plating efficiencies of 50-100% of total cell counts were obtained by optimizing inoculation method, H2S concentrations, and agar moisture content. The simple modifications required provide a readily available source of inexpensive anaerobic growth vessels for investigations requiring colonization of methanogens on solidified plating medium.

引用

页码：131 / 137

页数：7

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