Control of V(D)J recombinational accessibility of the Dβ1 gene segment at the TCRβ locus by a germline promoter

被引：122

作者：

Whitehurst, CE

Chattopadhyay, S

Chen, JZ ^{[1
]}

机构：

[1] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

IMMUNITY | 1999年 / 10卷 / 03期

关键词：

D O I：

10.1016/S1074-7613(00)80031-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The germline promoter region upstream of the D beta 1 gene segment in the murine TCR beta locus was deleted to assess its role in controlling V(D)J recombination. Associated with diminished D beta 1 region germline transcription, rearrangement of the D beta 1 but not the D beta 2 gene segment was reduced 10- to 20-fold. A corresponding reduction in RAG-mediated cleavage at the D beta 1 and J beta 1 signal sequences was apparent only when purified CD4(-)CD8(-) thymocytes were analyzed because, as we demonstrate, cleavage at these gene segments also occurred in CD4(+)CD8(+) thymocytes. These findings suggest that germline promoters regulate localized accessibility of gene segments for recombination and that in CD4(+)CD8(+) thymocytes TCR beta allelic exclusion does not result from inaccessibility of D beta gene segments.

引用

页码：313 / 322

页数：10

共 36 条

[21] TEMPORAL AND LINEAGE-SPECIFIC CONTROL OF T-CELL RECEPTOR ALPHA/DELTA-GENE REARRANGEMENT BY T-CELL RECEPTOR ALPHA-ENHANCER AND DELTA-ENHANCER [J].