In vitro assays for the determination of histone deacetylase activity

被引：97

作者：

Heltweg, B

Trapp, J

Jung, M

机构：

[1] Univ Freiburg, Dept Pharmaceut Sci, D-79104 Freiburg, Germany

[2] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA

来源：

METHODS | 2005年 / 36卷 / 04期

关键词：

historic deacetylase; sirtuin; assay; screening; aminomethylcoumarin;

D O I：

10.1016/j.ymeth.2005.03.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone deacetylases are important regulators of transcription and an emerging target for anticancer drugs. We present an overview over various assay formats that include radiolabelled histones, oligopeptides, and small molecules as substrates. The advantages and disadvantages of the various formats in terms of, e.g., substrate availability, throughput or subtype selectivity are discussed. Detailed procedures for various assay types that can be used for different problems, such as library screening or fluorescent inhibitor testing, are given. We present a new protocol for a simple high-throughput assay for NAD(+)-dependent (class III) histone deacetylases, also termed sirtuins. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：332 / 337

页数：6

共 23 条

[1] Apicidin: A novel antiprotozoal agent that inhibits parasite histone deacetylase [J].