ROS formation and glutathione levels in human oral fibroblasts exposed to TEGDMA and camphorquinone

被引:105
作者
Engelmann, J
Volk, J
Leyhausen, G
Geurtsen, W [1 ]
机构
[1] Univ Washington, Dept Restorat Dent, Div Operat Dent, Seattle, WA 98195 USA
[2] Hannover Med Sch, Dept Conservat Dent & Periodontol, D-30625 Hannover, Germany
关键词
pulp fibroblasts; TEGDMA; camphorquinone; reactive oxygen species; glutathione;
D O I
10.1002/jbm.b.30360
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Glutathione (GSH) is important for the self-protection of cells against oxidative stress and toxic xenobiotics, whereas reactive oxygen species (ROS) at elevated concentrations may cause detrimental alterations of cell membranes, DNA, and other cellular structures. The present investigation addressed the effects of triethylene-glycoldimethacrylate (TEGDMA) and camphorquinone (CQ) on glutathione metabolism and the formation of ROS in oral cells. Primary human pulp fibroblasts were exposed to various concentrations of TEGDMA and CQ (0.1-5 mM). Subsequently, GSH concentration and ROS formation were analyzed with the use of the monobromobimane assay (GSH) and 2',7'-dichlorofluorescein diacetate (DCFH-DA) (ROS). The endogenous ROS hydrogen peroxide (H2O2) was used as a positive control (0.02-2 mM). TEGDMA significantly decreased GSH at concentrations between 0.5 and 5 mM (P < 0.05), but did not elevate ROS levels. Contrary, CQ increased ROS formation at concentrations >= I mM, but had only a moderate effect on GSH at the highest test concentration. Hydrogen peroxide increased ROS and simultaneously decreased GSH at concentrations of a 0.2 mM. These data show that the investigated substances may cause cell damage due to various mechanisms, GSH decrease and/or ROS increase. As a consequence. TEGDMA and CQ released into an aqueous environment from resinous materials might interact, thus generating significant cytotoxic effects even at low concentrations. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:272 / 276
页数:5
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