Common secondary structures for the second internal transcribed spacer pre-rRNA of two subfamilies of trichostrongylid nematodes

被引:37
作者
Chilton, NB
Hoste, H
Newton, LA
Beveridge, I
Gasser, RB
机构
[1] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia
[2] INRA CR Tours, Pathol Aviaire & Parasitol Stn, F-37380 Nouzilly, France
基金
澳大利亚研究理事会;
关键词
ribosomal pre-rRNA; second internal transcribed spacer; nematodes; secondary structure model; Trichostrongylidae; Trichostrongylus; Camelostrongylus;
D O I
10.1016/S0020-7519(98)00129-5
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Sequences of the second internal transcribed spacer ribosomal DNA for the parasitic trichostrongylid nematodes Trichostrongylus probolurus, Trichostrongylus rugatus and Camelostrongylus mentulatus were compared with previously published sequences for five other species within the genus Trichostrongylus, The secondary structures of the second internal transcribed spacer pre-rRNA for these nematodes were predicted using an energy minimisation method. The results indicate that a common secondary structure of the second internal transcribed spacer of these nematodes is maintained despite distinct differences in primary sequence between species. Sequence differences among Trichostrongylus species ranged from 1.3 to 7.6%, but each species differed by 22-26% in sequence when compared with C. mentulatus which belongs to a different subfamily. (C) 1998 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1765 / 1773
页数:9
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