Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

被引：29

作者：

Studer, Bruno ^{[1
]}

Asp, Torben ^{[1
]}

Frei, Ursula ^{[1
]}

Hentrup, Stephan ^{[1
]}

Meally, Helena ^{[2
]}

Guillard, Aurelie ^{[2
]}

Barth, Susanne ^{[2
]}

Muylle, Hilde ^{[3
]}

Roldan-Ruiz, Isabel ^{[3
]}

Barre, Philippe ^{[4
]}

Koning-Boucoiran, Carole ^{[5
]}

Uenk-Stunnenberg, Gerda ^{[5
]}

Dolstra, Oene ^{[5
]}

Skot, Leif ^{[6
]}

Skot, Kirsten P. ^{[6
]}

Turner, Lesley B. ^{[6
]}

Humphreys, Mervyn O. ^{[6
]}

Kolliker, Roland ^{[7
]}

Roulund, Niels ^{[8
]}

Nielsen, Klaus K. ^{[8
]}

Lubberstedt, Thomas ^{[1
]}

机构：

[1] Univ Aarhus, Fac Agr Sci, Res Ctr Flakkebjerg, Dept Genet & Biotechnol, Slagelse 4200, Denmark

[2] TEAGASC, Crops Res Ctr Oak Pk, Carlow, Ireland

[3] Inst Agr & Fisheries Res ILVO, Plant Unit, Melle, Belgium

[4] INRA, Unite Genet & Ameliorat Plantes Fourrageres, Lusignan, France

[5] Plant Res Int BV, Wageningen, Netherlands

[6] Inst Grassland & Environm Res, Plant Genet & Breeding Dept, Aberystwyth, Dyfed, Wales

[7] Agroscope Reckenholz Tanikon Res Stn ART, Zurich, Switzerland

[8] TRIFOLIUM, DLF, Store Heddinge, Denmark

来源：

MOLECULAR BREEDING | 2008年 / 21卷 / 04期

关键词：

comparative mapping; expressed sequence tag (EST); ryegrass (Lolium spp.); simple sequence repeat (SSR);

D O I：

10.1007/s11032-007-9148-0

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

引用

页码：533 / 548

页数：16

共 20 条

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