Zinc transporter 3 immunohistochemical tracing of sprouting mossy fibres

被引：31

作者：

Chi, Zhi-Hong ^{[1
]}

Wang, Xin ^{[1
]}

Cai, Ji-Qun ^{[1
]}

Stoltenberg, Meredin ^{[2
]}

Danscher, Gorm ^{[2
]}

Wang, Zhan-You ^{[1
]}

机构：

[1] China Med Univ, Dept Histol & Embryol, Shenyang 110001, Peoples R China

[2] Univ Aarhus, Inst Anat, Dept Neurobiol, DK-8000 Aarhus C, Denmark

来源：

NEUROCHEMISTRY INTERNATIONAL | 2008年 / 52卷 / 07期

基金：

高等学校博士学科点专项科研基金; 中国博士后科学基金; 中国国家自然科学基金;

关键词：

zinc; zinc transporter 3; mossy fiber sprouting; autometallography; immunohistochemistry; mouse;

D O I：

10.1016/j.neuint.2008.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc transporter 3 (ZNT3) has been shown to transport zinc ions from the cytosol into presynaptic vesicles in the mammalian brain. Several studies have stated that the zinc ion containing synaptic vesicles of zinc-enriched neurons (ZEN) are loaded with ZNT3 proteins in their membranes. This fact makes it possible to trace sprouting mossy fibres in the temporal lobe epileptic hippocampus. In the present study, we examined the expression and distribution patterns of ZNT3 protein and chelatable zinc ions in the mouse hippocampus after pilocarpine treatment. Our results demonstrate that both ZNT3 immunostaining and autometallography reveal identical patterns of sprouting mossy fibres in the inner molecular layer in the mouse hippocampus. Using ZNT3 immuno-electron microscopic analysis we confirmed the presence of ectopic mossy fibre terminals in the inner molecular layer and found additionally by immuno-blotting a significant increase of ZNT3 in the pilocarpine-treated mouse hippocampi compared to age-matched controls. The increase of ZNT3 after pilocarpine treatment was time-dependent. The results support the notion that ZNT3 immunohistochemistry provides an excellent tool for tracing sprouting of ZEN terminals. The progressive increase of ZNT3 immunostaining in the temporal lobe epileptic hippocampus may relate to the increased levels of vesicular zinc ions during seizure. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1305 / 1309

页数：5

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