Changes in the vesicular zinc pattern following traumatic brain injury

被引:19
作者
Doering, P. [1 ]
Danscher, G. [1 ]
Larsen, A. [1 ]
Bruhn, M. [1 ]
Sondergaard, C. [1 ]
Stoltenberg, M. [1 ]
机构
[1] Univ Aarhus, Dept Neurobiol, DK-8000 Aarhus C, Denmark
关键词
AMG; nanocrystals; zinc ions; ZnSeAMG; ZnT3; brain lesion;
D O I
10.1016/j.neuroscience.2007.09.066
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The present study aims at evaluating the significance of zinc ions on the development of brain damage in a model of traumatic brain injury (TBI). The zinc ion specific autometallographic technique, the ZnSeAMG method, using silver enhancement of in vivo-captured zinc ions bound in zinc-selenium nanocrystals was applied to follow changes in the vesicular zinc pattern. Balb/c mice, ZnT3 knockout (ZnT3-Ko) mice, a mouse genetically knocked out for the protein ZnT3 responsible for sequestering zinc into synaptic vesicles, and littermates from the genetically un-manipulated mother type mice, wild type (Wt), were used. The Wt and the Balb/c mice exhibited instantaneously a boost in the zinc staining adjacent to the lesion involving all six neocortical layers. Ultra-structural analyses revealed that the in vivo created ZnSe nanocrystals were still confined to the vesicles of the zinc-enriched (ZEN) neurons in the neuropil. No differences between the Balb/c and Wt mice were seen at any time points. In the ZnT3-Ko mice the ZEN terminals stayed void of AMG grains, but a number of neuronal somata around the lesion became loaded with ZnSe nanocrystals. These silver-enhanced ZnSe nanocrystals were confined to the cytoplasm of the somata and their proximal dendrites. No such soma staining was seen in the Wt or Balb/c mice. We speculate that vesicular zinc may not contribute to neuronal damage following TBI. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:93 / 103
页数:11
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