共 67 条
Changes in the vesicular zinc pattern following traumatic brain injury
被引:19
作者:
Doering, P.
[1
]
Danscher, G.
[1
]
Larsen, A.
[1
]
Bruhn, M.
[1
]
Sondergaard, C.
[1
]
Stoltenberg, M.
[1
]
机构:
[1] Univ Aarhus, Dept Neurobiol, DK-8000 Aarhus C, Denmark
关键词:
AMG;
nanocrystals;
zinc ions;
ZnSeAMG;
ZnT3;
brain lesion;
D O I:
10.1016/j.neuroscience.2007.09.066
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
The present study aims at evaluating the significance of zinc ions on the development of brain damage in a model of traumatic brain injury (TBI). The zinc ion specific autometallographic technique, the ZnSeAMG method, using silver enhancement of in vivo-captured zinc ions bound in zinc-selenium nanocrystals was applied to follow changes in the vesicular zinc pattern. Balb/c mice, ZnT3 knockout (ZnT3-Ko) mice, a mouse genetically knocked out for the protein ZnT3 responsible for sequestering zinc into synaptic vesicles, and littermates from the genetically un-manipulated mother type mice, wild type (Wt), were used. The Wt and the Balb/c mice exhibited instantaneously a boost in the zinc staining adjacent to the lesion involving all six neocortical layers. Ultra-structural analyses revealed that the in vivo created ZnSe nanocrystals were still confined to the vesicles of the zinc-enriched (ZEN) neurons in the neuropil. No differences between the Balb/c and Wt mice were seen at any time points. In the ZnT3-Ko mice the ZEN terminals stayed void of AMG grains, but a number of neuronal somata around the lesion became loaded with ZnSe nanocrystals. These silver-enhanced ZnSe nanocrystals were confined to the cytoplasm of the somata and their proximal dendrites. No such soma staining was seen in the Wt or Balb/c mice. We speculate that vesicular zinc may not contribute to neuronal damage following TBI. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.
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页码:93 / 103
页数:11
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