High concordance of daidzein-metabolizing phenotypes in individuals measured 1 to 3 years apart

被引:71
作者
Frankenfeld, CL
Atkinson, C
Thomas, WK
Gonzalez, A
Jokela, T
Wähälä, K
Schwartz, SM
Li, SS
Lampe, JW
机构
[1] Fred Hutchinson Canc Res Ctr, Canc Prevent Program, Seattle, WA 98109 USA
[2] Univ Washington, Dept Epidemiol, Seattle, WA USA
[3] Univ Washington, Interdisciplinary Program Nutr Sci, Seattle, WA USA
[4] Univ Helsinki, Dept Chem, Helsinki, Finland
[5] Fred Hutchinson Canc Res Ctr, Epidemiol Program, Seattle, WA USA
关键词
equol; O-desmethylangolensin; soya; daidzein;
D O I
10.1079/BJN20051565
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Particular intestinal bacteria are capable of metabolizing the soya isoflavone daidzein to equol and/or O-desmethylangolensin (O-DMA), and the presence of these metabolites in urine after soya consumption are markers of particular intestinal bacteria profiles. Prevalences of equol producers and O-DMA producers are approximately 30-50 % and 80-90 %, respectively, and limited observations have suggested that these daidzein-metabolizing phenotypes are stable within individuals over time. Characterizing stability of these phenotypes is important to understand their potential as markers of long-term exposure to particular intestinal bacteria and their associations with disease risk. We evaluated concordance within an individual for the equol-producer and O-DMA-producer phenotypes measured at two time points (T1, T2), 1-3 years apart. Phenotypes were ascertained by analysing equol and O-DMA using GC-MS in a spot urine sample collected after 3 d soya (source of daidzein) supplementation. In ninety-two individuals without recent (within 3 months before phenotyping) or current antibiotics use, 41 % were equol producers at T1 and 45 % were equol producers at T2, and 90 % were O-DMA producers at T1 and 95 % were O-DMA producers at T2. The percentage agreement for the equol-producer phenotype was 82 and for the O-DMA-producer phenotype was 89. These results indicate that these phenotypes are stable in most individuals over time, suggesting that they provide a useful biomarker for evaluating disease risk associated with harbouring particular intestinal bacteria responsible for, or associated with, the metabolism of the soya isoflavone daidzein.
引用
收藏
页码:873 / 876
页数:4
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