The role of biomaterials in the direction of mesenchymal stem cell properties and extracellular matrix remodelling in dermal tissue engineering

被引:72
作者
Schneider, Rebekka K. [1 ]
Anraths, Julia [1 ]
Kramann, Rafael [2 ]
Bornemann, Joerg [3 ]
Bovi, Manfred [3 ]
Knuechel, Ruth [1 ]
Neuss, Sabine [1 ]
机构
[1] Rhein Westfal TH Aachen, Fac Med, Inst Pathol, D-52074 Aachen, Germany
[2] Rhein Westfal TH Aachen, Fac Med, Div Nephrol & Clin Immunol, D-52074 Aachen, Germany
[3] Rhein Westfal TH Aachen, Fac Med, Elect Microscop Facil, D-52074 Aachen, Germany
关键词
Dermal tissue engineering; Mesenchymal stem cells; Organotypic skin culture; Matrix remodelling; Paracrine activity; EPIDERMAL MORPHOGENESIS; HACAT KERATINOCYTES; EXPRESSION; DIFFERENTIATION; ORGANIZATION; EQUIVALENTS; MECHANISMS; DISEASE; GROWTH; REPAIR;
D O I
10.1016/j.biomaterials.2010.07.003
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Recently, a new generation of dermal equivalents (DE) was presented which are solely generated on a human fibroblast-derived dermal matrix. Since human mesenchymal stem cells from bone marrow (BM-MSC) and Wharton's Jelly of the umbilical cord (UC-MSC) are characterised by a distinct biosynthetic and paracrine activity, they are an appealing alternative approach for generating cell-based DE. This study compares the epithelial-mesenchymal interaction and extracellular matrix (ECM) remodelling of cell-based and collagen-based DE using fibroblasts, BM-MSC or UC-MSC, respectively, in co-culture with the keratinocyte cell line HaCaT. While fibroblast-based DE exhibit normal matrix synthesis, proliferation and differentiation of keratinocytes, mesenchymal stem cell-based DE resulted in excessive production of inhomogenous matrix aggregates, loss of polarisation of the epidermal cell layer and an inconstant paracrine activity. In contrast, collagen-embedded MSC revealed a homogenous growth pattern as well as a constant expression of growth factors and ECM proteins without a negative influence on the epidermal layer as shown by histology, electron microscopy, immunohistochemistry and realtime-RT-PCR. These results indicate the necessity of an instructive biomaterial-based scaffold to direct stem cell differentiation, proliferation, paracrine activity as well as regulation of ECM deposition. (c) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:7948 / 7959
页数:12
相关论文
共 36 条
[1]   EPIDERMAL MORPHOGENESIS AND INDUCTION OF THE 67-KD KERATIN POLYPEPTIDE BY CULTURE OF HUMAN KERATINOCYTES AT THE LIQUID AIR INTERFACE [J].
ASSELINEAU, D ;
BERNHARD, B ;
BAILLY, C ;
DARMON, M .
EXPERIMENTAL CELL RESEARCH, 1985, 159 (02) :536-539
[2]   Potential risks of bone marrow cell transplantation into infarcted hearts [J].
Breitbach, Martin ;
Bostani, Toktam ;
Roell, Wilhelm ;
Xia, Ying ;
Dewald, Oliver ;
Nygren, Jens M. ;
Fries, Jochen W. U. ;
Tiemann, Klaus ;
Bohlen, Heribert ;
Hescheler, Juergen ;
Welz, Armin ;
Bloch, Wilhelm ;
Jacobsen, Sten Eirik W. ;
Fleischmann, Bernd K. .
BLOOD, 2007, 110 (04) :1362-1369
[3]  
BREITKREUTZ D, 1991, CANCER RES, V51, P4402
[4]   Why are MSCs therapeutic? New data: new insight [J].
Caplan, Al .
JOURNAL OF PATHOLOGY, 2009, 217 (02) :318-324
[5]   All MSCs are pericytes? [J].
Caplan, Arnold I. .
CELL STEM CELL, 2008, 3 (03) :229-230
[6]   A perivascular origin for mesenchymal stem cells in multiple human organs [J].
Crisan, Mihaela ;
Yap, Solomon ;
Casteilla, Louis ;
Chen, Chien-Wen ;
Corselli, Mirko ;
Park, Tea Soon ;
Andriolo, Gabriella ;
Sun, Bin ;
Zheng, Bo ;
Zhang, Li ;
Norotte, Cyrille ;
Teng, Pang-Ning ;
Traas, Jeremy ;
Schugar, Rebecca ;
Deasy, Bridget M. ;
Badylak, Stephen ;
Buehring, Hans-Joerg ;
Giacobino, Jean-Paul ;
Lazzari, Lorenza ;
Huard, Johnny ;
Peault, Bruno .
CELL STEM CELL, 2008, 3 (03) :301-313
[7]   Adult stem cells and repair through granulation tissue [J].
Diaz-Flores, Lucio, Jr. ;
Gutierrez, Ricardo ;
Madrid, Juan Francisco ;
Varela, Hilda ;
Valladares, Francisco ;
Diaz-Flores, Lucio .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2009, 14 :1433-1470
[8]   Replacement of animal-derived collagen matrix by human fibroblast-derived dermal matrix for human skin equivalent products [J].
El Ghalbzouri, Abdoelwaheb ;
Commandeur, Suzan ;
Rietveld, Marion H. ;
Mulder, Aat A. ;
Willemze, Rein .
BIOMATERIALS, 2009, 30 (01) :71-78
[9]   GROWTH AND DIFFERENTIATION CHARACTERISTICS OF TRANSFORMED KERATINOCYTES FROM MOUSE AND HUMAN-SKIN INVITRO AND INVIVO [J].
FUSENIG, NE ;
BREITKREUTZ, D ;
DZARLIEVA, RT ;
BOUKAMP, P ;
BOHNERT, A ;
TILGEN, W .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1983, 81 (01) :S168-S175
[10]   β1 Integrin Cytoplasmic Domain Residues Selectively Modulate Fibronectin Matrix Assembly and Cell Spreading through Talin and Akt-1 [J].
Green, J. Angelo ;
Berrier, Allison L. ;
Pankov, Roumen ;
Yamada, Kenneth M. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (12) :8148-8159