Chloroplast NADH dehydrogenase from Pisum sativum:: Characterization of its activity and cloning of ndhK gene

被引：11

作者：

Elortza, F ^{[1
]}

Asturias, JA ^{[1
]}

Arizmendi, JM ^{[1
]}

机构：

[1] Euskal Herriko Unibertsitatea, Biokimika & Biol Mol Saila, Bilbao 48080, Spain

来源：

PLANT AND CELL PHYSIOLOGY | 1999年 / 40卷 / 02期

关键词：

chlororespiration; complex I; cyclic electron transport; NAD(P)H dehydrogenase (EC 1.6.99.3); ndh gene; Pisum sativum;

D O I：

10.1093/oxfordjournals.pcp.a029522

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The pea chloroplast ndhK gene coding for a component of a NADH-plastoquinone oxidoreductase has been cloned and sequenced. This gene codes for a polypeptide of 227 amino acids and a predicted molecular mass of 25,495 Da which belongs to the family of the 20 kDa PSST subunit of the bovine mitochondrial complex I. A fragment of this gene has been overexpressed in Escherichia coli, and antibodies against the expressed polypeptide recognize a protein of the predicted molecular mass from pea thylakoid membranes. This polypeptide is a component of a protein complex with NADH dehydrogenase activity and is not associated with ferredoxin-NADP(+) reductase.

引用

页码：149 / 154

页数：6

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