Biotransformation of chlormadinone acetate to delmadinone acetate by free and immobilized Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805

被引：10

作者：

Manosroi, J ^{[1
]}

Sripalakit, P ^{[1
]}

Manosroi, A ^{[1
]}

机构：

[1] Chiang Mai Univ, Fac Pharm, Div Pharmaceut Sci, Chiang Mai 50200, Thailand

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2003年 / 33卷 / 2-3期

关键词：

Arthrobacter simplex; Bacillus sphaericus; biotransformation; chlormadinone acetate; delmadinone acetate; liposomes; biphasic;

D O I：

10.1016/S0141-0229(03)00161-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial transformation of chlormadinone acetate to delmadinone acetate was investigated using free and immobilized cells of Arthrobacter simplex ATCC 6946 and Bacillus sphaericus ATCC 13805 in liquid-liquid biphasic system and a liposomal medium. For liquid-liquid biphasic system, n-decane, n-octanol, chloroform and butyl acetate were used as organic solvents. In liposomal medium, chlormadinone acetate was entrapped in liposomes composed of phosphatidylcholine and cholesterol. For both immobilized and free suspension biocatalysts, yields were higher in the aqueous medium compared to in liquid-liquid biphasic media. In all cases, the highest yields were obtained in the aqueous system. For the aqueous medium, a product yield of similar to48% was attained at 3 h using the free suspension A. simplex cells. The aqueous medium afforded similar to47% product yield at 48 h when freely suspended B. sphaericus was used. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：320 / 325

页数：6

共 20 条

[1] CYPROTERONE - A REVIEW OF ITS PHARMACOLOGY AND THERAPEUTIC EFFICACY IN PROSTATE-CANCER [J].

BARRADELL, LB ;

FAULDS, D .

DRUGS & AGING, 1994, 5 (01) :59-80

[2] A STRUCTURE-ACTIVITY RELATIONSHIP STUDY OF SPIROLACTONES - CONTRIBUTION OF THE CYCLOPROPANE RING TO ANTIMINERALOCORTICOID ACTIVITY [J].

CHINN, LJ ;

SALAMON, KW ;

DESAI, BN .

JOURNAL OF MEDICINAL CHEMISTRY, 1981, 24 (09) :1103-1107

[3]

EVANS TW, 1998, Patent No. 4749679

[4] BIOCONVERSION OF A HYDROCORTISONE DERIVATIVE IN AN ORGANIC-AQUEOUS 2-LIQUID-PHASE SYSTEM [J].

FERNANDES, P ;

CABRAL, JMS ;

PINHEIRO, HM .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (02) :163-167

[5] DEHYDROGENATION OF HYDROCORTISONE BY ARTHROBACTER-SIMPLEX IN A LIPOSOMAL MEDIUM [J].

GOETSCHEL, R ;

BAR, R .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (03) :245-251

[6]

GOODHUE CT, 1986, MANUAL IND MICROBIOL, P98

[7]

HOCKNULL MD, 1990, APPL MICROBIOL BIOT, V33, P148

[8] EFFECT OF SUPERSATURATED AQUEOUS HYDROCORTISONE CONCENTRATIONS ON THE DELTA-1-DEHYDROGENASE ACTIVITY OF FREE AND IMMOBILIZED ARTHROBACTER-SIMPLEX [J].

KLOOSTERMAN, J ;

LILLY, MD .

ENZYME AND MICROBIAL TECHNOLOGY, 1984, 6 (03) :113-116

[9] ON OPTIMIZING ORGANIC-SOLVENTS IN MULTI-LIQUID-PHASE BIOCATALYSIS [J].

LAANE, C ;

BOEREN, S ;

VOS, K .

TRENDS IN BIOTECHNOLOGY, 1985, 3 (10) :251-252

[10]

LEDNICER D, 1980, ORGANIC CHEM DRUG SY, V2, P166

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