DEHYDROGENATION OF HYDROCORTISONE BY ARTHROBACTER-SIMPLEX IN A LIPOSOMAL MEDIUM

被引：15

作者：

GOETSCHEL, R ^{[1
]}

BAR, R ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM, DEPT APPL MICROBIOL, POB 1172, IL-91010 JERUSALEM, ISRAEL

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 03期

关键词：

LIPOSOME; MICROBIAL CATALYSIS; ARTHROBACTER; HYDROCORTISONE;

D O I：

10.1016/0141-0229(91)90136-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biotransformation of hydrocortisone to prednisolone by Arthrobacter simplex ATCC 6946 was extensively investigated in a liposomal medium, made up of multilamellar vesicles of either pure or crude egg phospholipids. The liposomal medium was found to promote faster kinetics to a higher conversion than those obtained in an aqueous medium and to be superior to water/surfactant and water/cosolvent systems, especially at high substrate loadings. The enhancing effect of the liposomal medium was partly explained by its capacity for steroid entrapment as well as by the presence of phospholipid vesicles in the bioconversion medium.

引用

页码：245 / 251

页数：7

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