Metabolic characteristics of an isocitrate dehydrogenase defective derivative of Escherichia coli BL21(DE3)

被引:22
作者
Aoshima, M
Ishii, M
Yamagishi, A
Oshima, T
Igarashi, Y
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan
[2] Tokyo Univ Pharm & Life Sci, Dept Mol Biol, Hachioji, Tokyo 1920355, Japan
关键词
isocitrate dehydrogenase; isocitrate lyase; citrate synthase; citrate accumulation; Escherichia coli BL21 (DE3);
D O I
10.1002/bit.10832
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A 7.8 kb fragment containing isocitrate dehydrogenase (ICDH) gene and its flanking regions was cloned from Escherichia coli BL21(DE3) and sequenced. Unlike the case of the K-12 strain, the e14 element was not found. The nucleotide divergence between these two strains was about 2%. Using the cloned fragment, ICDH defective mutant strain, MA1935, was generated from BL21(DE3). Although MA1935 accumulated citrate, citrate synthase activity was not repressed but was rather high. In addition, isocitrate lyase was not highly induced at the stationary phase. MA1935 was shown to be a good host strain for ICDH gene expression. (C) 2003 Wiley Periodicals Inc.
引用
收藏
页码:732 / 737
页数:6
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