Novel association between sperm deformity index and oxidative stress-induced DNA damage in infertile male patients

被引:54
作者
Said, TM
Aziz, N
Sharma, RK
Lewis-Jones, I
Thomas, AJ
Agarwal, A
机构
[1] Cleveland Clin Fdn, Ctr Adv Res Human Reprod Infertil & Sexual Funct, Glickman Urol Inst, Cleveland, OH 44195 USA
[2] Cleveland Clin Fdn, Dept Obstet & Gynecol, Cleveland, OH 44195 USA
[3] Liverpool Womens Hosp, Reprod Med Unit, Liverpool L87SSS, Merseyside, England
关键词
beta-nicotinamide adenine dinucleotide phosphate; oxidative stress; sperm deformity index; sperm DNA damage;
D O I
10.1111/j.1745-7262.2005.00022.x
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Aim: To investigate the impact of abnormal sperm morphology using the sperm deformity index (SDI) on reactive oxygen species (ROS) production and its correlation with sperm DNA damage. Methods: Semen samples were collected from men undergoing infertility screening (n = 7) and healthy donors (n = 6). Mature spermatozoa were isolated and incubated with 5 mmol/L beta-nicotinamide adenine dinucleotide phosphate (NADPH) for up to 24 It to induce ROS. Sperm morphology was evaluated using strict Tygerberg's criteria and the SDI. ROS levels and DNA damage were assessed using chemiluminescence and terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling (TUNEL) assays, respectively. Results: SDI values (median [interquartiles]) were higher in patients than donors (2 [1.8, 2.1] vs. 1.53 [1.52, 1.58], P = 0.008). Aliquots treated with NADPH showed higher ROS levels (1.22 [0.30, 1.87] vs. 0.39 [0.10, 0.57], P = 0.03) and higher incidence of DNA damage than those not treated (10 [4.69, 24.85] vs. 3.85 [2.58, 5.10], P = 0.008). Higher DNA damage was also seen following 24 h of incubation in patients compared to donors. SDI correlated with the percentage increase in sperm DNA damage following incubation for 24 h in samples treated with NADPH (r = 0.7, P = 0.008) and controls (r = 0.58, P = 0.04). Conclusion: SDI may be a useful tool in identifying potential infertile males with abnormal prevalence of oxidative stress(OS)-induced DNA damage. NADPH plays a role in ROS-mediated sperm DNA damage, which appears to be more evident in infertile patients with semen samples containing a high incidence of morphologically abnormal spermatozoa.
引用
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页码:121 / 126
页数:6
相关论文
共 24 条
  • [11] 2-K
  • [12] GORCZYCA W, 1993, CANCER RES, V53, P1945
  • [13] GROW DR, 1994, FERTIL STERIL, V62, P559
  • [14] NEW METHOD OF EVALUATING SPERM MORPHOLOGY WITH PREDICTIVE VALUE FOR HUMAN INVITRO FERTILIZATION
    KRUGER, TF
    ACOSTA, AA
    SIMMONS, KF
    SWANSON, RJ
    MATTA, JF
    VEECK, LL
    MORSHEDI, M
    BRUGO, S
    [J]. UROLOGY, 1987, 30 (03) : 248 - 251
  • [15] Genomic imprinting in disruptive spermatogenesis
    Marques, CJ
    Carvalho, F
    Sousa, M
    Barros, A
    [J]. LANCET, 2004, 363 (9422) : 1700 - 1702
  • [16] Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds
    Menkveld, R
    Wong, WY
    Lombard, CJ
    Wetzels, AMM
    Thomas, CMG
    Merkus, HMWM
    Steegers-Theunissen, RPM
    [J]. HUMAN REPRODUCTION, 2001, 16 (06) : 1165 - 1171
  • [17] Muratori M, 2000, J ANDROL, V21, P903
  • [18] Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility
    Ollero, M
    Gil-Guzman, E
    Lopez, MC
    Sharma, RK
    Agarwal, A
    Larson, K
    Evenson, D
    Thomas, AJ
    Alvarez, JG
    [J]. HUMAN REPRODUCTION, 2001, 16 (09) : 1912 - 1921
  • [19] The sperm deformity and the sperm multiple anomalies indexes in patients who underwent unilateral orchectomy and preventive radiotherapy
    Panidis, D
    Matalliotakis, I
    Papathanasiou, K
    Roussos, C
    Koumantakis, E
    [J]. EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1998, 80 (02): : 247 - 250
  • [20] Saleh RA, 2002, J ANDROL, V23, P737